From DrugPedia: A Wikipedia for Drug discovery

Rv2163c
Name
Penicillin-binding membrane protein PbpB
Identifiers
Swiss Prot	O06214
Genbank	887949
PDB	?
Chemical data
Formula	C19H16N2O4 [1]
Mol. wt.	72506 Da
Pharmacokinetic data
Bioavailability	?
Solubility	?
Isoelectric-Point	10.0838

Contents 1 General 1.1 Protein Sequence 2 Human Homologue Blast Result 3 Alergen Protein 4 Bacterial Toxin Prediction 5 Subcellular Location 6 Antigens 6.1 B cell Epitopes 6.1.1 BCEpred Analysis 6.1.2 ABCpred Analysis 6.1.3 IEDB Analysis 7 MHC Class-I Binder 7.1 nHLAPred Analysis 7.2 IEDB Analysis 8 MHC Class-II Binder 8.1 Propred Analysis 8.2 NetMHC-II Analysis 9 External Links

[edit] General

Involved in peptidoglycan biosynthesis, Probable penicillin-binding membrane protein pbpB

Rv2163c, (MTCY270.05), len: 679 aa. Probable pbpB, penicillin-binding membrane protein, similar to many bacterial PBP2 proteins e.g. P11882|PBP2_NEIME|PENA|NMA2072|NMB0413 penicillin-binding protein 2 (pbp-2) from Neisseria meningitidis (serogroups A and B) (581 aa), FASTA scores: opt: 665, E(): 1.6e-31, (33.2% identity in 591 aa overlap); etc. Also similar to Rv0016c and Rv2864c from Mycobacterium tuberculosis (2.8e-10). Contains PS00017 possible ATP/GTP-binding site motif A (P-loop) near C-terminus. FASTA best: PBP2_NEIME P11882 penicillin-binding protein 2 (pbp-2). (581 aa) opt: 665, E(): 1.6e-31; (33.2% identity in 591 aa overlap)

Essential gene by Himar1-based transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003) mutants available at TARGET website

[edit] Protein Sequence

>Rv2163c, TB.seq 2425049:2427085 MW:72506 VSRAAPRRASQSQSTRPARGLRRPPGAQEVGQRKRPGKTQKARQAQEATKSRPATRSDVAPAGRSTRARRTRQVVDVGTR GASFVFRHRTGNAVILVLMLVAATQLFFLQVSHAAGLRAQAAGQLKVTDVQPAARGSIVDRNNDRLAFTIEARALTFQPK RIRRQLEEARKKTSAAPDPQQRLRDIAQEVAGKLNNKPDAAAVLKKLQSDETFVYLARAVDPAVASAICAKYPEVGAERQ DLRQYPGGSLAANVVGGIDWDGHGLLGLEDSLDAVLAGTDGSVTYDRGSDGVVIPGSYRNRHKAVHGSTVVLTLDNDIQF YVQQQVQQAKNLSGAHNVSAVVLDAKTGEVLAMANDNTFDPSQDIGRQGDKQLGNPAVSSPFEPGSVNKIVAASAVIEHG LSSPDEVLQVPGSIQMGGVTVHDAWEHGVMPYTTTGVFGKSSNVGTLMLSQRVGPERYYDMLRKFGLGQRTGVGLPGESA GLVPPIDQWSGSTFANLPIGQGLSMTLLQMTGMYQAIANDGVRVPPRIIKATVAPDGSRTEEPRPDDIRVVSAQTAQTVR QMLRAVVQRDPMGYQQGTGPTAGVPGYQMAGKTGTAQQINPGCGCYFDDVYWITFAGIATADNPRYVIGIMLDNPARNSD GAPGHSAAPLFHNIAGWLMQRENVPLSPDPGPPLVLQAT

[edit] Human Homologue Blast Result

subject ids	% identity	% positives	alignment length	evalue
sp|P07196.3	22	41	234	2.4
sp|Q9P225.3	31	57	61	2.7
sp|Q8TD26.3	24	50	70	3.1
sp|Q5T601.1	24	44	58	3.1
sp|P08670.4	21	39	180	4.7

[edit] Alergen Protein

Link to Algpred
Non Allergen Predicted by AlgPred Server

[edit] Bacterial Toxin Prediction

Link to btxpred
No Hit Fountd by btxpred server.

[edit] Subcellular Location

Link to TBpred
Integral Membrane

[edit] Antigens

No Hit Found in AntigenDB
No. Hit Found in IEDB

[edit] B cell Epitopes

[edit] BCEpred Analysis

Link to Bcepred
Result Predicited by [2]

[edit] ABCpred Analysis

Link to ABCpred
Result Predicited by [3]

[edit] IEDB Analysis

Link to IEDB
Result Predicited by [4]

[edit] MHC Class-I Binder

[edit] nHLAPred Analysis

Link to nHLApred
Result Predicited by [5]

[edit] IEDB Analysis

Link to IEDB
Result Predicted by [6]

[edit] MHC Class-II Binder

[edit] Propred Analysis

Link to Propred
Result Predicted by [7]

[edit] NetMHC-II Analysis

Link to NetMHC-II
Result Predicted by [8]

[edit] External Links

• Database of Mycobacterium tuberculosis genome sequences and related information.