CarboDB Lipopolysaccharide 2510
From DrugPedia: A Wikipedia for Drug discovery
[edit] Carbohydrate Name
Lipopolysaccharide
[edit] Carbohydrate Class
Lipopolysaccharide
[edit] Source Microbe
Vibrio cholerae 01 serogroup
[edit] Basic Structure
The O-antigen part consists of (1-->2)-α-linked 4-amino-4,6-dideoxy-D-mannose (perosamine) whose amino group is acylated with 3-deoxy-L-glycero-tetronic acid. The terminal sugar in the Inaba O-antigen has a hydroxyl at the 2' position
[edit] Proposed functions
V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
[edit] Antigenic Nature used to produce antibodies
Killed Vaccine consisting of V. cholerae O1 Inaba (ATCC 14033) and Ogawa (ATCC 14035) cells
[edit] Carrier Name
nil
[edit] Conjugation Method
nil
[edit] Antibodies
Mab A19
[edit] Antibody type and class
IgM
[edit] Assay System
enzyme-linked immunosorbent assay (ELISA), immunofluorescence, Immunoelectron microscopy and the slide agglutination test
[edit] Cross-reactivity
This Mab reacted with both Inaba and Ogawa serovars (A antigen) and was specific to O1 serotype. It however reacted weakly with five V. cholerae non-O1 serovars and Serratia marcescens
[edit] Proposed epitopes
The epitope was named as Epitope A. Epitope A was postulated to be either the perosamine residues or the N-tetronic acid (N-3-deoxy-L-glycero-tetronic acid) side chain
[edit] Proposed Utility
The A19 anti-A antigen MAb agglutinated each O1 serovar and Serratia marcescens, albeit weakly
