CarboDB O-polysaccharide 2056

From DrugPedia: A Wikipedia for Drug discovery

Contents 1 Carbohydrate Name 2 Carbohydrate Class 3 Source Microbe 4 Basic Structure 5 Proposed functions 6 Antigenic Nature used to produce antibodies 7 Carrier Name 8 Conjugation Method 9 Antibodies 10 Antibody type and class 11 Assay System 12 Cross-reactivity 13 Proposed epitopes 14 Proposed Utility 15 Weblink 16 External Links

Carbohydrate Name

O-polysaccharide

Carbohydrate Class

O-polysaccharide [LPS]

Source Microbe

Pseudomonas syringae IMV 7836 pv. Atrofaciens

Basic Structure

O-polysaccharides of the LPS consists of tetra- and tri-α-D-rhamnose repeats in the backbone [-3)D-Rha(α1->3)D-Rha(α1-->2)D-Rha(α1->2)D-Rha(α1] and [3)D-Rha(α1-->3)D-Rha(α1-->2)D-Rha(α1-]

Proposed functions

N/A

Antigenic Nature used to produce antibodies

Whole cells

Carrier Name

nil

Conjugation Method

nil

Antibodies

Mab Ps1a'

Antibody type and class

IgG3

Assay System

Agglutination, Ouchterlony immunodiffusion, ELISA, SDS-PAGE, and Western immunoblotting

Cross-reactivity

"Mab Ps1a' cross-reacted with all strains from subgroups 1b, 1c, and 1x but not with strains from 1d."

Proposed epitopes

The epitope of Mab1a' is related to the D-Rha(alpha1-3)D-Rha(alpha1-2)D-Rha site of the O-polysaccharide backbone consisting of tetra-D-rhamnose repeats. The residue (alpha1-3)DRha( alpha1-2) is immunodominant for epitope of Mab 1a'

Proposed Utility

This Mab was useful for elucidation of the LPs structure

Weblink

PubMed Central

External Links

• Link to CarboDB database