CarboDB Core oligosaccharide 2022

From DrugPedia: A Wikipedia for Drug discovery

Contents 1 Carbohydrate Name 2 Carbohydrate Class 3 Source Microbe 4 Basic Structure 5 Proposed functions 6 Antigenic Nature used to produce antibodies 7 Carrier Name 8 Conjugation Method 9 Antibodies 10 Antibody type and class 11 Assay System 12 Cross-reactivity 13 Proposed epitopes 14 Proposed Utility 15 Weblink 16 External Links

Carbohydrate Name

Core oligosaccharide

Carbohydrate Class

Core oligosaccharide [LPS]

Source Microbe

Pseudomonas aeruginosa

Basic Structure

The LPS consists of three regions, namely O-polysaccharide, core oligosaccharide and lipid A. The core region of LPS is divided into the outer core and inner core. The inner core region consists of 2-keto-3-deoxyoctonic acid (KDO), heptose, phosphate, and probably ethanolamine. The outer core region commonly contains D-glucose, L-rhamnose, D-galactosamine, and L-alanine. The O-antigens consist of a trisaccharide repeating unit which consists of two uronic acid derivatives and one N-acetyl fucosamine residue

Proposed functions

N/A

Antigenic Nature used to produce antibodies

formalin-killed P. aeruginosa cells

Carrier Name

nil

Conjugation Method

nil

Antibodies

Mab MH-4H7

Antibody type and class

IgM

Assay System

ELISA, immunoblotting and flow cytometry

Cross-reactivity

Mab MH-4H7 bound to P. aeruginosa strains of serotype A, F, G, H, K and M, but did not bind to any strains of serotype B, C, E and I. This Mab also cross-reacted with E. coli 026 : B6 LPS

Proposed epitopes

This Mab reacted with the core region of the LPS. L-rhamnose in the LPS core of serotype G was an immunodominant sugar recognized by OM-1D6

Proposed Utility

This Mab showed a high level of protective activity against P.aeruginosa in an experimental infection model using normal mice

Weblink

PubMed

External Links

• Link to CarboDB database