From DrugPedia: A Wikipedia for Drug discovery

Immunitation of Rv2220
Name
Glutamine synthetase GLNA1 (glutamine synthase) (GS-I)
Identifiers
Swiss Prot	Q10377
Genbank	888383
PDB	?
Chemical data
Formula	?
Mol. wt.	53569.5
Pharmacokinetic data
Bioavailability	?
Solubility	?
Isoelectric-Point	4.84

Contents 1 General 1.1 Protein Sequence 2 Human Homologue Blast Result 3 Alergen Protein 4 Bacterial Toxin Prediction 5 Subcellular Location 6 Antigens 6.1 B cell Epitopes 6.1.1 BCEpred Analysis 6.1.2 ABCpred Analysis 6.1.3 IEDB Analysis 7 MHC Class-I Binder 7.1 nHLAPred Analysis 7.2 IEDB Analysis 8 MHC Class-II Binder 8.1 Propred Analysis 8.2 NetMHC-II Analysis 9 External Links

[edit] General

The gene glnA1 is held cardinal as it codes for the enzyme glutamine synthase which play a vital role in inducing pathogenesis to M.tuberculosis.This enzyme also plays a significant part in nitrogen metabolism.Mycobacterium tuberculosis possess four Glutamine synthetase homologues, two of which, glnA1 and glnA2 are required for virulence and are located on the bacterial chromosome on either side of glutamine synthetase adenylyltransferase (glnE). To assess the role of glutamine synthetase,a glnA1 mutant was constructed via allelic exchange. When complemented by the M. tuberculosis glnA1 gene, the mutant exhibited a wild-type phenotype in broth culture and in human macrophages, and it was virulent in guinea pigs.The major GS(Glutamine synthase) of Mycobacterium tuberculosis is GlnA1, a type I GS whose activity is controlled by posttranscriptional modification by GlnE. Glutamine synthetase catalyzes the ATP-dependent condensation of glutamate and ammonia to form glutamine.Structural basis for the inhibition of Mycobacterium tuberculosis glutamine synthetase IS by the use of novel ATP-competitive inhibitors.Temperature-sensitive mutants of GlnE were generated and shown to have a defect in growth and GS activity in nitrogen-limited medium.Virulence was unaffected in both in vitro and in vivo model systems of infection, indicating that the presence of extra-cellular GS is not critical for virulence and that the residual intra-cellular GS activity is sufficient.

[edit] Protein Sequence

>Rv2220, TB.seq 2487613:2489046 MW:53539 VTEKTPDDVFKLAKDEKVEYVDVRFCDLPGIMQHFTIPASAFDKSVFDDGLAFDGSSIRGFQSIHESDMLLLPDPETARI DPFRAAKTLNINFFVHDPFTLEPYSRDPRNIARKAENYLISTGIADTAYFGAEAEFYIFDSVSFDSRANGSFYEVDAISG WWNTGAATEADGSPNRGYKVRHKGGYFPVAPNDQYVDLRDKMLTNLINSGFILEKGHHEVGSGGQAEINYQFNSLLHAAD DMQLYKYIIKNTAWQNGKTVTFMPKPLFGDNGSGMHCHQSLWKDGAPLMYDETGYAGLSDTARHYIGGLLHHAPSLLAFT NPTVNSYKRLVPGYEAPINLVYSQRNRSACVRIPITGSNPKAKRLEFRSPDSSGNPYLAFSAMLMAGLDGIKNKIEPQAP VDKDLYELPPEEAASIPQTPTQLSDVIDRLEADHEYLTEGGVFTNDLIETWISFKRENEIEPVNIRPHPYEFALYYDV

[edit] Human Homologue Blast Result

subject ids	% identity	% positives	alignment length	evalue
sp|Q5TDP6	23	40	504	2e-17
sp|P15104	25	41	162	7e-04
sp|Q15058	27	38	77	0.51
sp|Q96FW1	29	48	54	5.0
sp|P56962	27	46	47	6.6

[edit] Alergen Protein

Link to Algpred
Allergen Predicted by AlgPred Server

[edit] Bacterial Toxin Prediction

Link to btxpred
No Hit Fountd by btxpred server.

[edit] Subcellular Location

Link to TBpred
Cyoplasmic

[edit] Antigens

No Hit Found in AntigenDB
No. Hit Found in IEDB

[edit] B cell Epitopes

[edit] BCEpred Analysis

Link to Bcepred
Result Predicited by [1]

[edit] ABCpred Analysis

Link to ABCpred
Result Predicited by [2]

[edit] IEDB Analysis

Link to IEDB
Result Predicited by [3]

[edit] MHC Class-I Binder

[edit] nHLAPred Analysis

Link to nHLApred
Result Predicited by [4]

[edit] IEDB Analysis

Link to IEDB
Result Predicted by [5]

[edit] MHC Class-II Binder

[edit] Propred Analysis

Link to Propred
Result Predicted by [6]

[edit] NetMHC-II Analysis

Link to NetMHC-II
Result Predicted by [7]

[edit] External Links

• Database of Mycobacterium tuberculosis genome sequences and related information.