Immunotation of Rv2753c (dihydrodipicolinate synthase)

From DrugPedia: A Wikipedia for Drug discovery

Revision as of 07:09, 5 January 2010 by Deepak (Talk | contribs)
Jump to: navigation, search
Immunotation of Rv2753c (dihydrodipicolinate synthase)
Name
Dihydrodipicolinate Synthase
Identifiers
Swiss Prot P63945
Genbank 888289
PDB 1XXX
Chemical data
Formula C28H37ClN4O6 [1]
Mol. wt. 30,858 Da
Pharmacokinetic data
Bioavailability  ?
Solubility  ?
Isoelectric-Point 5.57

Contents

General

dapA catalyzes the formation of dihydrodipicolinate from L-aspartate 4-semialdehyde and pyruvate.

L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2H2O.

This protein is eesential for optimal growth of M.tb and found in the cytoplasm as predicted by the WoLFPSORT and Subloc Server. HMMpfam found one domain PF00701 and has Helix Turn Helix secondary structure.

Protein Sequence

>Rv2753c, TB.seq 3066222:3067121 MW:30827 VTTVGFDVAARLGTLLTAMVTPFSGDGSLDTATAARLANHLVDQGCDGLVVSGTTGESPTTTDGEKIELLRAVLEAVGDR ARVIAGAGTYDTAHSIRLAKACAAEGAHGLLVVTPYYSKPPQRGLQAHFTAVADATELPMLLYDIPGRSAVPIEPDTIRA LASHPNIVGVKDAKADLHSGAQIMADTGLAYYSGDDALNLPWLAMGATGFISVIAHLAAGQLRELLSAFGSGDIATARKI NIAVAPLCNAMSRLGGVTLSKAGLRLQGIDVGDPRLPQVAATPEQIDALAADMRAASVLR

Human Homologue Blast Result

subject ids% identity% positivesalignment length evalue
sp|Q9BXD5 28 46.33 300 3.00E-023
sp|Q86XE5 26.57 46.15 286 4.00E-022
sp|Q9UG63 26.49 37.75 151 0.45
sp|O14556 22.09 50 86 2
sp|Q7Z7B0 27.69 44.62 65 2.7

Antigens

Epitopes

B-Cell Epitopes

BCEpred

Result Predicted by bcelpep  
Result Predicited by Predicited by ABCPred]
Result Predicted by IEDB Result Predicited by Predicited by IEDB]

PFSGDGSLDTA

GTTGESPTTTDGE

GTYDTAH

AEG

YSKPPQRGLQ

DATEL

RSAVPIEPDTIRA

KADLHSG

YSGDDALN

IATAR

GDPRLPQVAATPEQIDALAAD

MHC class I restricted T cell epitopes

CTL Epitopes

MHC Binder

Class-I Binder

Result Predicited by Predicited by nHLApred
Result Predicted by nHLApred

EpitopeNo of Alleles
VPIEPDTIR 15
TPFSGDGSL 14
TLSKAGLRL 13
IEPDTIRAL 13
GTYDTAHSI 13
DDALNLPWL 11
GFISVIAHL 10
GATGFISVI 10
ATPEQIDAL 10
YDTAHSIRL 9
GDIATARKI 9
AVLEAVGDR 9
ASHPNIVGV 9
QRGLQAHFT 7
GLQAHFTAV 7
AVGDRARVI 7
YSGDDALNL 6
VGFDVAARL 6
QLRELLSAF 6
TAVADATEL 5
TAARLANHL 5
LLRAVLEAV 5
GEKIELLRA 5
DTATAARLA 5
AAGQLRELL 5
TDGEKIELL 4
QAHFTAVAD 4
MSRLGGVTL 4
LDTATAARL 4
HPNIVGVKD 4
GVKDAKADL 4
DGEKIELLR 4
KIELLRAVL 3
HSIRLAKAC 3
GSGDIATAR 3
AHLAAGQLR 3
PTTTDGEKI 2
IAHLAAGQL 2
DLHSGAQIM 2

Class-II Binder

Result predicted by NetMHCII

EpitopeNo of Alleles
TGFISVIAHLAAGQL 6
GFISVIAHLAAGQLR 6
PDTIRALASHPNIVG 4
GATGFISVIAHLAAG 4
ATGFISVIAHLAAGQ 4
TELPMLLYDIPGRSA 3
MGATGFISVIAHLAA 3
LVVTPYYSKPPQRGL 3
GQLRELLSAFGSGDI 3
FISVIAHLAAGQLRE 3
DTIRALASHPNIVGV 3
QLRELLSAFGSGDIA 2
PEQIDALAADMRAAS 2
NLPWLAMGATGFISV 2
LPWLAMGATGFISVI 2
LNLPWLAMGATGFIS 2
LLVVTPYYSKPPQRG 2
LGGVTLSKAGLRLQG 2
KAGLRLQGIDVGDPR 2
IAGAGTYDTAHSIRL 2
GLLVVTPYYSKPPQR2
GGVTLSKAGLRLQGI 2
EPDTIRALASHPNIV 2
ELPMLLYDIPGRSAV 2
ATELPMLLYDIPGRS 2
ALNLPWLAMGATGFI 2
AGQLRELLSAFGSGD 2
AGLRLQGIDVGDPRL 2

Result Predicited by Propred
Epitope No of Alleles
IRLAKACAA 51
INIAVAPLC 51
VVSGTTGES 48
LLVVTPYYS 48
IRALASHPN 46
LTAMVTPFS 44
LQAHFTAVA 43
LGTLLTAMV 43
LLTAMVTPF 42
VAPLCNAMS 41
IATARKINI 40
LRELLSAFG 38
LLYDIPGRS 38
LVVSGTTGE 37
VIAHLAAGQ 36
MLLYDIPGR 36
LRLQGIDVG 35
ISVIAHLAA 34
LQGIDVGDP 33
FISVIAHLA 33
WLAMGATGF 32
LLRAVLEAV 30
IELLRAVLE 30
YYSKPPQRG 25
IAVAPLCNA 24
LAADMRAAS 22
IVGVKDAKA 22
LCNAMSRLG 21
LANHLVDQG 21
LAMGATGFI 21
FGSGDIATA 20
YSGDDALNL 19
VGFDVAARL 18
LRAVLEAVG 17
LGGVTLSKA 17
IEPDTIRAL 17
LVVTPYYSK 16
LSKAGLRLQ 16
LHSGAQIMA 16
IMADTGLAY 16
IAHLAAGQL 16
VGVKDAKAD 15
MVTPFSGDG 15
LASHPNIVG 15
FSGDGSLDT 14
LNLPWLAMG 13
VGDRARVIA 12
IAGAGTYDT 11
FDVAARLGT 11
IPGRSAVPI 10
VTPYYSKPP 9
MSRLGGVTL 9
LSAFGSGDI 9
YDTAHSIRL 8
VTLSKAGLR 6
MGATGFISV 5
LYDIPGRSA 5
LVDQGCDGL 5
LAYYSGDDA 5
VAARLGTLL 4
LDTATAARL 4
VTPFSGDGS 3
VPIEPDTIR 3
LLSAFGSGD 3
LAKACAAEG 3
YSKPPQRGL 2
YDIPGRSAV 2
VVTPYYSKP 2
LPQVAATPE 2
LEAVGDRAR 2
LAAGQLREL 2

Alergen Protein

Non Allergen Predicted by AlgPred Server

Antibacterial

Bacterial Toxin

External Links