From DrugPedia: A Wikipedia for Drug discovery

Revision as of 08:00, 17 December 2009 by Deepak (Talk | contribs)

(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)

Immunotation of Rv2753c (dihydrodipicolinate synthase)
Name
Dihydrodipicolinate Synthase
Identifiers
Swiss Prot	P63945
Genbank	888289
PDB	1XXX
Chemical data
Formula	?
Mol. wt.	30,858 Da
Pharmacokinetic data
Bioavailability	?
Solubility	?
Isoelectric-Point	5.57

General

dapA catalyzes the formation of dihydrodipicolinate from L-aspartate 4-semialdehyde and pyruvate.

L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2H₂O.

This protein is eesential for optimal growth of M.tb and found in the cytoplasm as predicted by the WoLFPSORT and Subloc Server. HMMpfam found one domain PF00701 and has Helix Turn Helix secondary structure.

Human Homologue Blast Result

subject ids	% identity	% positives	alignment length	evalue
sp\|Q9BXD5	28	46.33	300	3.00E-023
sp\|Q86XE5	26.57	46.15	286	4.00E-022
sp\|Q9UG63	26.49	37.75	151	0.45
sp\|O14556	22.09	50	86	2
sp\|Q7Z7B0	27.69	44.62	65	2.7

Antigens

Haptens

Epitopes

B-Cell Epitopes

PFSGDGSLDTA

GTTGESPTTTDGE

GTYDTAH

AEG

YSKPPQRGLQ

DATEL

RSAVPIEPDTIRA

KADLHSG

YSGDDALN

IATAR

GDPRLPQVAATPEQIDALAAD

MHC class I restricted T cell epitopes

CTL Epitopes

MHC Binder

Class-I Binder

Epitope	No of Alleles
VPIEPDTIR	15
TPFSGDGSL	14
TLSKAGLRL	13
IEPDTIRAL	13
GTYDTAHSI	13
DDALNLPWL	11
GFISVIAHL	10
GATGFISVI	10
ATPEQIDAL	10
YDTAHSIRL	9
GDIATARKI	9
AVLEAVGDR	9
ASHPNIVGV	9
QRGLQAHFT	7
GLQAHFTAV	7
AVGDRARVI	7
YSGDDALNL	6
VGFDVAARL	6
QLRELLSAF	6
TAVADATEL	5
TAARLANHL	5
LLRAVLEAV	5
GEKIELLRA	5
DTATAARLA	5
AAGQLRELL	5
TDGEKIELL	4
QAHFTAVAD	4
MSRLGGVTL	4
LDTATAARL	4
HPNIVGVKD	4
GVKDAKADL	4
DGEKIELLR	4
KIELLRAVL	3
HSIRLAKAC	3
GSGDIATAR	3
AHLAAGQLR	3
PTTTDGEKI	2
IAHLAAGQL	2
DLHSGAQIM	2

Class-II Binder

Result predicted by NetMHCII

Epitope	No of Alleles
TGFISVIAHLAAGQL	6
GFISVIAHLAAGQLR	6
PDTIRALASHPNIVG	4
GATGFISVIAHLAAG	4
ATGFISVIAHLAAGQ	4
TELPMLLYDIPGRSA	3
MGATGFISVIAHLAA	3
LVVTPYYSKPPQRGL	3
GQLRELLSAFGSGDI	3
FISVIAHLAAGQLRE	3
DTIRALASHPNIVGV	3
QLRELLSAFGSGDIA	2
PEQIDALAADMRAAS	2
NLPWLAMGATGFISV	2
LPWLAMGATGFISVI	2
LNLPWLAMGATGFIS	2
LLVVTPYYSKPPQRG	2
LGGVTLSKAGLRLQG	2
KAGLRLQGIDVGDPR	2
IAGAGTYDTAHSIRL	2
GLLVVTPYYSKPPQR	2
GGVTLSKAGLRLQGI	2
EPDTIRALASHPNIV	2
ELPMLLYDIPGRSAV	2
ATELPMLLYDIPGRS	2
ALNLPWLAMGATGFI	2
AGQLRELLSAFGSGD	2
AGLRLQGIDVGDPRL	2

</td></tr>

Epitope: No of Alleles

IRLAKACAA 51

INIAVAPLC 51

VVSGTTGES 48

LLVVTPYYS 48

IRALASHPN 46

LTAMVTPFS 44

LQAHFTAVA 43

LGTLLTAMV 43

LLTAMVTPF 42

VAPLCNAMS 41

IATARKINI 40

LRELLSAFG 38

LLYDIPGRS 38

LVVSGTTGE 37

VIAHLAAGQ 36

MLLYDIPGR 36

LRLQGIDVG 35

ISVIAHLAA 34

LQGIDVGDP 33

FISVIAHLA 33

WLAMGATGF 32

LLRAVLEAV 30

IELLRAVLE 30

YYSKPPQRG 25

IAVAPLCNA 24

LAADMRAAS 22

IVGVKDAKA 22

LCNAMSRLG 21

LANHLVDQG 21

LAMGATGFI 21

FGSGDIATA 20

YSGDDALNL 19

VGFDVAARL 18

LRAVLEAVG 17

LGGVTLSKA 17

IEPDTIRAL 17

LVVTPYYSK 16

LSKAGLRLQ 16

LHSGAQIMA 16

IMADTGLAY 16

IAHLAAGQL 16

VGVKDAKAD 15

MVTPFSGDG 15

LASHPNIVG 15

FSGDGSLDT 14

LNLPWLAMG 13

VGDRARVIA 12

IAGAGTYDT 11

FDVAARLGT 11

IPGRSAVPI 10

VTPYYSKPP 9

MSRLGGVTL 9

LSAFGSGDI 9

YDTAHSIRL 8

VTLSKAGLR 6

MGATGFISV 5

LYDIPGRSA 5

LVDQGCDGL 5

LAYYSGDDA 5

VAARLGTLL 4

LDTATAARL 4

VTPFSGDGS 3

VPIEPDTIR 3

LLSAFGSGD 3

LAKACAAEG 3

YSKPPQRGL 2

YDIPGRSAV 2

VVTPYYSKP 2

LPQVAATPE 2

LEAVGDRAR 2

LAAGQLREL 2

Alergen Protein

Non Allergen Predicted by AlgPred Server

Antibacterial

Bacterial Toxin

External Links

Immunotation of Rv2753c (dihydrodipicolinate synthase)