PolysacDB O-polysaccharide 2564

From DrugPedia: A Wikipedia for Drug discovery

Contents 1 Carbohydrate Name 2 Carbohydrate Class 3 Source Microbe 4 Basic Structure 5 Proposed functions 6 Antigenic Nature used to produce antibodies 7 Carrier Name 8 Conjugation Method 9 Antibodies 10 Antibody type and class 11 Assay System 12 Cross-reactivity 13 Proposed epitopes 14 Proposed Utility 15 Weblink 16 External Links

Carbohydrate Name

O-polysaccharide

Carbohydrate Class

O-polysaccharide [LPS]

Source Microbe

Xanthomonas hyacinthi S148

Basic Structure

The Xanthomonas LPS showed a predominance of mannose (Man), glucose (Glc), galacturonic acid (GalA), and N-acetylglucosamine (GlcNAc) residues

Proposed functions

N/A

Antigenic Nature used to produce antibodies

crude cell wall preparation and purified fimbriae

Carrier Name

nil

Conjugation Method

nil

Antibodies

Mab 2E5

Antibody type and class

IgM

Assay System

immunoblotting, immunofluorescence, enzyme-linked immunosorbent assay (ELISA), and immunoelectron microscopy

Cross-reactivity

This Mab cross-reacted with all X. hyacinthi strains. No cross-reaction was found with 27 other Xanthomonas pathovars tested or with 14 other bacterial species from other genera, such as Erwinia and Pseudomonas

Proposed epitopes

The O antigen was found to consist of rhamnose and fucose in a 2:1 molar ratio. Mab 2E5 recognized only S-LPS and did not recognize R-LPS

Proposed Utility

This Mab could be useful for structural studies, for detection purposes in commercial and agricultural settings, and for serological typing purposes

Weblink

PubMed

External Links

• Link to PolysacDB database