PolysacDB O-polysaccharide 2058
From DrugPedia: A Wikipedia for Drug discovery
Carbohydrate Name
O-polysaccharide
Carbohydrate Class
O-polysaccharide [LPS]
Source Microbe
Pseudomonas syringae IMV K-1025 pv. Atrofaciens
Basic Structure
O-polysaccharides of the LPS consists of tetra- and tri-α-D-rhamnose repeats in the backbone [-3)D-Rha(α1->3)D-Rha(α1-->2)D-Rha(α1->2)D-Rha(α1] and [3)D-Rha(α1-->3)D-Rha(α1-->2)D-Rha(α1-]
Proposed functions
N/A
Antigenic Nature used to produce antibodies
Whole cells
Carrier Name
nil
Conjugation Method
nil
Antibodies
Mab Ps1b
Antibody type and class
IgM
Assay System
Agglutination, Ouchterlony immunodiffusion, ELISA, SDS-PAGE, and Western immunoblotting
Cross-reactivity
" P. syringae strains with pathovars glycinea, japonica, striafaciens, atrofaciens and 2 P. syringae strains for which the pathovar(s) has not been identified cross-reacted strongly with the MAb Ps1c"
Proposed epitopes
The epitope was confined to lateral immunodominant substituents of (α1-->3)D-Rha, (β1-->4)D-GlcNAc, and (α1-->4)D-Fuc, respectively, which are all bound to the backbone-located D-rhamnose
Proposed Utility
This Mab strongly agglutinated homologous bacterial cells and precipitated soluble antigens