PolysacDB O-polysaccharide 2055
From DrugPedia: A Wikipedia for Drug discovery
Carbohydrate Name
O-polysaccharide
Carbohydrate Class
O-polysaccharide [LPS]
Source Microbe
Pseudomonas syringae IMV 7836 pv. Atrofaciens
Basic Structure
O-polysaccharides of the LPS consists of tetra- and tri-α-D-rhamnose repeats in the backbone [-3)D-Rha(α1->3)D-Rha(α1-->2)D-Rha(α1->2)D-Rha(α1] and [3)D-Rha(α1-->3)D-Rha(α1-->2)D-Rha(α1-]
Proposed functions
N/A
Antigenic Nature used to produce antibodies
Whole cells
Carrier Name
nil
Conjugation Method
nil
Antibodies
Mab Ps1a
Antibody type and class
IgG3
Assay System
Agglutination, Ouchterlony immunodiffusion, ELISA, SDS-PAGE, and Western immunoblotting
Cross-reactivity
"All P. syringae strains with pathovars aptata, glycinea, japonica, phaseolicola, and pisi as well as 72 (87.8%), 3 (75%), and 31 (50%) strains with pathovars atrofaciens, striafaciens, and syringae, respectively, and 20 (80%) P. syringae strains for which the pathovar(s) has not been identified cross-reacted strongly with the MAb Ps1a"
Proposed epitopes
The epitope of Mab 1a is related to the Rha(α1-->2)D-Rha(α1-->3)D-Rha sequence of the O-polysaccharide backbone with the immunodominant site (α1-->2)D-rhamnose(α1-->3)
Proposed Utility
This Mab was useful for elucidation of the LPs structure
