PolysacDB Marine fungal polysaccharide YCP 1911
From DrugPedia: A Wikipedia for Drug discovery
Carbohydrate Name
Marine fungal polysaccharide YCP
Carbohydrate Class
Miscellaneous
Source Microbe
Phoma herbarum YS4108
Basic Structure
It has a structure of α-(1-->4)-D-glucan branched with α-(1-->6)-D-linked side chain
Proposed functions
YCP strongly stimulates both cell-mediated and humoral immunities in in vivo and in vitro conditions. YCP administration could significantly suppress the growth of mouse-transplanted tumor
Antigenic Nature used to produce antibodies
Glycoconjugates
Carrier Name
Bovine serum albumin
Conjugation Method
80 ul of 1-cyano dimethylamino pyridinium tetrafluorate (400 mM in acetonitrile) was added to 2 ml of YCP (4 uM in water). The pH of the reaction mixture was subsequently raised to 9.0 by the addition of 300 mM triethylamine. After 80 ul of BSA (2 mM in 150 mM NaCl) being added, the reaction lasting overnight at C was quenched with 1 ml of 500 mM ethanolamine. The reaction mixture was dialyzed against 10 mM PBS (pH 7.4) and then applied to a Sephacryl S400 column (100 4 1.6 cm) eluted with PBS. The fractions containing the conjugate were pooled, dialyzed against distilled water. The amount of polysaccharide in the conjugate was determined by the sulfuric acid method and the content of protein by s method. Its hapten ratio was estimated as mole of protein per mole of polysaccharide. The conjugate emerged as a single peak in the void volume and entirely separated from free BSA and/or YCP
Antibodies
Mab N3F
Antibody type and class
IgM
Assay System
inhibition ELISA, double immunodiffusion
Cross-reactivity
This Mab was highly specific to P. herbarum YCP polysaccharide
Proposed epitopes
N/A
Proposed Utility
This Mab is a promising drug candidate
