PolysacDB Lipooligosaccharide 1616
From DrugPedia: A Wikipedia for Drug discovery
Carbohydrate Name
Lipooligosaccharide
Carbohydrate Class
Lipopolysaccharide
Source Microbe
Moraxella catarrhalis
Basic Structure
LOS consists of an oligosaccharide and lipid A and is similar to the lipopolysaccharide (LPS) of gramnegative enteric pathogens, but it lacks the O-antigenic side chain of repeating units characteristic of classical LPS. The oligosaccharide part consists of the following residues : α-D-Galp-(1-->4)-β-D-Galp-(1-->4)-α-D-Glcp-(1-->2)-β-D-Glcp-(1-->6)-α-D-Glcp [branched to α-D-GlcpNAc-(1-->2)-β-D-Glcp-(1-->4)] and [branched to β-D-Glcp-(1-->3)] -(1-->5)-Kdo[ branched to Kdop-(2-?)]
Proposed functions
Moracella lipooligosaccharide is a potential virulence factor
Antigenic Nature used to produce antibodies
Whole cells
Carrier Name
nil
Conjugation Method
nil
Antibodies
Mab 8E7
Antibody type and class
IgG3
Assay System
ELISA, immunoblotting, Bacterial adherence assay, Bactericidal assay and immunoelectron microscopy
Cross-reactivity
MAb 8E7 reacted with M. catarrhalis O35E, serotype A and C LOSs but not serotype B LOS
Proposed epitopes
The epitope recognized by MAb 8E7 is directed to a common sequence of either α-GlcNAc-(1-->2)-β-Glc-(1--> at the branch substituting position 4 of the trisubstituted Glc residue or a terminal tetrasaccharide α-Gal-(1-->4)-β-Gal-(1-->4)-α-Glc-(1-->2)-β-Glc-[1--> at the branch substituting position 6 of the trisubstituted Glc residue
Proposed Utility
This Mab had had bactericidal activity. Passive immunization with Mab 8E7 could significantly enhance the clearance of strain O35E from mouse lungs in an aerosol challenge mouse model
