PolysacDB Lipopolysaccharide 1164
From DrugPedia: A Wikipedia for Drug discovery
Carbohydrate Name
Lipopolysaccharide
Carbohydrate Class
Lipopolysaccharide
Source Microbe
Bordetella pertusis 1414
Basic Structure
B. pertusis LPS is resolved electrophoretically into two bands, a slowly migrating band designated LOS A and a fast-migrating band designated LOS B. These two species differ in molecular weight as three N-acetyl amino sugars found on LOS A are not present on LOS B
Proposed functions
The LOS of B. pertussis exhibits biological activities similar to those of endotoxins from other gram-negative bacteria, including pyrogenicity, toxicity, induction of the Shwartzman reaction, induction of tumor necrosis factor production, and adjuvant properties
Antigenic Nature used to produce antibodies
B. pertusis LPS
Carrier Name
nil
Conjugation Method
nil
Antibodies
Mab P1P3
Antibody type and class
IgM
Assay System
ELISA and Immunostaining
Cross-reactivity
This Mab did not cross-react with the B. pertusis variant strain A100 but cross-reacted with a B. bronchiseptica LPS and B. parapertussis LPS
Proposed epitopes
The epitope was proposed to be terminal sugar units nonacetylated glucosamine substituted with L-glycero-b-manno-heptose, present in the 'core' of the B. pertussis LPS. The carbon atoms 3,4, and 6 of this glucosamine unit could be involved in the epitope
Proposed Utility
This Mab can be useful for rapid typing of Bordetella in clinical isolates and can be used to detect antigenic modifications in LPSs from variant B. pertassis strains, or from related Bordetella species (B. parapertassis, B. bronchiseptica
