CarboDB Capsular polysaccharide 2602

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Contents

Carbohydrate Name

Capsular polysaccharide

Carbohydrate Class

Capsular polysaccharide

Source Microbe

Cryptococcus neoformans Serotype D

Basic Structure

The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated


Proposed functions

The capsular polysaccharide is an important virulence factor

Antigenic Nature used to produce antibodies

Glycoconjugates

Carrier Name

Sheep erythrocytes

Conjugation Method

The capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature

Antibodies

Mab 386

Antibody type and class

N/A

Assay System

ELISA and Immunodiffusion

Cross-reactivity

This antibody has a highly variable cross-reactivity. It cross reacts with most strains of Serotype A and D. It cross reacts with only one strain of serotype B and 2 strains of serotype C. does not show a reaction with ELISA

Proposed epitopes

Since Mab 386 does not show any positive results with ELISA, it is suggested that binding of polysaccharides to plates coated with poly-L-lysine altered the carboxyl residues that may form part of an epitope recognized by the antibody

Proposed Utility

used to differentiate various strains of all serotypes

Weblink

PubMed

External Links