CarboDB Pneumococcal vaccine PP 2466

From DrugPedia: A Wikipedia for Drug discovery

Contents 1 Carbohydrate Name 2 Carbohydrate Class 3 Source Microbe 4 Basic Structure 5 Proposed functions 6 Antigenic Nature used to produce antibodies 7 Carrier Name 8 Conjugation Method 9 Antibodies 10 Antibody type and class 11 Assay System 12 Cross-reactivity 13 Proposed epitopes 14 Proposed Utility 15 Weblink 16 External Links

Carbohydrate Name

Pneumococcal vaccine PP

Carbohydrate Class

O-polysaccharide [LPS]

Source Microbe

Streptococcus pneumoniae

Basic Structure

This vaccine includes the polysaccharides of 6B, 14, 19F, 18C, 23F serotypes

Proposed functions

N/A

Antigenic Nature used to produce antibodies

Glycoconjugates

Carrier Name

Diphtheria toxin mutant (CRM197)

Conjugation Method

The oligosaccharide-CRM197 conjugates were synthesized by either first hydrolysing the native oligosaccharide [PS] to smaller fragments and then subjecting the fragments to limited periodate oxidation or by oxidizing the native PS and then hydrolyzing it into smaller fragments. The PS-CRM197 conjugates were synthesized by oxidizing native pneumococcal PS with sodium metaperiodate to introduce random aldehyde functional groups. Oxidized OS or PS fragments were coupled to CRM197 in the presence of sodium cyanoborohydride

Antibodies

Human Antisera

Antibody type and class

N/A

Assay System

ELISA, Opsonophagocytic killing assay

Cross-reactivity

The antisera reacted with 6B serotype, showed good opsonophagocytic activity for 19F serotype. This antisera also cross-reacted with 6A serotype but did not react with 19A serotype

Proposed epitopes

N/A

Proposed Utility

The findings suggest that some conjugate vaccines may elicit cross-protection against related polysaccharides better than others

Weblink

PubMed

External Links

• Link to CarboDB database