CarboDB Lipopolysaccharide 1571

From DrugPedia: A Wikipedia for Drug discovery

Contents 1 Carbohydrate Name 2 Carbohydrate Class 3 Source Microbe 4 Basic Structure 5 Proposed functions 6 Antigenic Nature used to produce antibodies 7 Carrier Name 8 Conjugation Method 9 Antibodies 10 Antibody type and class 11 Assay System 12 Cross-reactivity 13 Proposed epitopes 14 Proposed Utility 15 Weblink 16 External Links

Carbohydrate Name

Lipopolysaccharide

Carbohydrate Class

Lipopolysaccharide

Source Microbe

Leptospira interrogans serovar pomona

Basic Structure

The oligosaccharide contained rhamnose, ribose, glucose, and glucosamine

Proposed functions

N/A

Antigenic Nature used to produce antibodies

Glycoconjugates

Carrier Name

Diphtheria toxoid

Conjugation Method

The oligosaccharide was modified as follows : it was dissolved in N-ethylmorpholine-water-12 N HCl (2.5:6.5:1 [vol/vol/vol], pH 8.5) in concentrations of approximately 24 umol in 250ul. 125umol of N-succinimidyl S-acetylmercaptoacetate in 250ul of N,N dimethylacetamide was added to the oligosaccharide. This solution was added to modified diphtheria toxoid

Antibodies

Polysera

Antibody type and class

IgG and IgM

Assay System

Enzyme Immunoassay, immunoblotting and dot blot

Cross-reactivity

This antisera cross-reacted with LPS from some other leptospiral serovars (serovars hardjobovis, ballum, copenhageni, canicola, grippotyphosa, and batavia) and LPS from other bacteria (Salmonella typhimurium and the Re mutant of Shigella flexneri) and did not react with serovars australis, grippotyphosa, ballum, or tarassovi

Proposed epitopes

N/A

Proposed Utility

This antiserum induced a high CL response in mouse macrophage monolayers

Weblink

PubMed Central

External Links

• Link to CarboDB database