CarboDB O-polysaccharide 2278
From DrugPedia: A Wikipedia for Drug discovery
[edit] Carbohydrate Name
O-polysaccharide
[edit] Carbohydrate Class
O-polysaccharide [LPS]
[edit] Source Microbe
Shigella flexneri serotype 4a
[edit] Basic Structure
Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->
[edit] Proposed functions
LPS plays an important virulence role based on its antiphagocytic and endotoxic properties
[edit] Antigenic Nature used to produce antibodies
Whole cells
[edit] Carrier Name
nil
[edit] Conjugation Method
nil
[edit] Antibodies
Mab MASF IV-2
[edit] Antibody type and class
IgG2c
[edit] Assay System
ELISA
[edit] Cross-reactivity
This Mab cross-reacted with serotype 4b. The MASF IV-2 antibodies agglutinated the two 4b strains, 14 of 20 4a strains (among them the strain that was negative for MASF IV-1), and 19 of 21 serotype 4 strains
[edit] Proposed epitopes
α-D-Glcp-1-->6-β-D-GlcpN residues seemed to be the part of the epitope
[edit] Proposed Utility
This Mab may prove to be valuable reagents in the studies of protective immunity against Shigella bacteria
