PolysacDB Capsular polysaccharide 2605
From DrugPedia: A Wikipedia for Drug discovery
Revision as of 06:14, 8 April 2010
Carbohydrate Name
Capsular polysaccharide
Carbohydrate Class
Capsular polysaccharide
Source Microbe
Cryptococcus neoformans Serotype D
Basic Structure
The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
Proposed functions
The capsular polysaccharide is an important virulence factor
Antigenic Nature used to produce antibodies
Glycoconjugates
Carrier Name
Keyhole limpet hemocyanin
Conjugation Method
Forty milligrams of crude polysaccharide was dissolved in 4 ml of physiological saline solution (PSS). One milliliter of 1% cyanuric chloride in dimethylformamide was added to the polysaccharide solution and stirred for 1 h at 48C. KLH in PSS (100 mg/2ml) was added and stirred for 1 h at 258C, and the solution was then incubated at 48C for 18 h. The solution was dialyzed against water for 30 h and centrifuged. The protein concentration in the supernatant was adjusted to 2 mg/ml, 1/9 volume of 10% aluminum potassium sulfate solution was added, and the pH was adjusted to 6.5 with 10% sodium carbonate. The precipitated antigen was removed by centrifugation and resuspended in PSS
Antibodies
Mab CRND8
Antibody type and class
IgM
Assay System
ELISA and CSA[Cell Slide Agglutination]
Cross-reactivity
This antibody cross-reacted with Serotype A and D serotypes
Proposed epitopes
O-acetylated glucurunoxylomannan
Proposed Utility
used to differentiate various strains of all serotypes
