PolysacDB Capsular polysaccharide 2602
From DrugPedia: A Wikipedia for Drug discovery
Revision as of 06:14, 8 April 2010
Carbohydrate Name
Capsular polysaccharide
Carbohydrate Class
Capsular polysaccharide
Source Microbe
Cryptococcus neoformans Serotype D
Basic Structure
The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
Proposed functions
The capsular polysaccharide is an important virulence factor
Antigenic Nature used to produce antibodies
Glycoconjugates
Carrier Name
Sheep erythrocytes
Conjugation Method
The capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature
Antibodies
Mab 386
Antibody type and class
N/A
Assay System
ELISA and Immunodiffusion
Cross-reactivity
This antibody has a highly variable cross-reactivity. It cross reacts with most strains of Serotype A and D. It cross reacts with only one strain of serotype B and 2 strains of serotype C. does not show a reaction with ELISA
Proposed epitopes
Since Mab 386 does not show any positive results with ELISA, it is suggested that binding of polysaccharides to plates coated with poly-L-lysine altered the carboxyl residues that may form part of an epitope recognized by the antibody
Proposed Utility
used to differentiate various strains of all serotypes
