PolysacDB O-polysaccharide 2564

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Contents 1 Carbohydrate Name 2 Carbohydrate Class 3 Source Microbe 4 Basic Structure 5 Proposed functions 6 Antigenic Nature used to produce antibodies 7 Carrier Name 8 Conjugation Method 9 Antibodies 10 Antibody type and class 11 Assay System 12 Cross-reactivity 13 Proposed epitopes 14 Proposed Utility 15 Weblink 16 External Links

[edit] Carbohydrate Name

O-polysaccharide

[edit] Carbohydrate Class

O-polysaccharide [LPS]

[edit] Source Microbe

Xanthomonas hyacinthi S148

[edit] Basic Structure

The Xanthomonas LPS showed a predominance of mannose (Man), glucose (Glc), galacturonic acid (GalA), and N-acetylglucosamine (GlcNAc) residues

[edit] Proposed functions

N/A

[edit] Antigenic Nature used to produce antibodies

crude cell wall preparation and purified fimbriae

[edit] Carrier Name

nil

[edit] Conjugation Method

nil

[edit] Antibodies

Mab 2E5

[edit] Antibody type and class

IgM

[edit] Assay System

immunoblotting, immunofluorescence, enzyme-linked immunosorbent assay (ELISA), and immunoelectron microscopy

[edit] Cross-reactivity

This Mab cross-reacted with all X. hyacinthi strains. No cross-reaction was found with 27 other Xanthomonas pathovars tested or with 14 other bacterial species from other genera, such as Erwinia and Pseudomonas

[edit] Proposed epitopes

The O antigen was found to consist of rhamnose and fucose in a 2:1 molar ratio. Mab 2E5 recognized only S-LPS and did not recognize R-LPS

[edit] Proposed Utility

This Mab could be useful for structural studies, for detection purposes in commercial and agricultural settings, and for serological typing purposes

[edit] Weblink

PubMed

[edit] External Links

• Link to PolysacDB database