PolysacDB Lipopolysaccharide 2528
From DrugPedia: A Wikipedia for Drug discovery
Revision as of 06:14, 8 April 2010
Carbohydrate Name
Lipopolysaccharide
Carbohydrate Class
Lipopolysaccharide
Source Microbe
Vibrio cholerae 01 serogroup Inaba strain X19203
Basic Structure
The O-antigen part consists of (1-->2)-α-linked 4-amino-4,6-dideoxy-D-mannose (perosamine) whose amino group is acylated with 3-deoxy-L-glycero-tetronic acid. The terminal sugar in the Inaba O-antigen has a hydroxyl at the 2' position
Proposed functions
V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodies
Killed cells
Carrier Name
nil
Conjugation Method
nil
Antibodies
Mab IH 15
Antibody type and class
IgG3
Assay System
slide agglutination, microdilution agglutination, and passive hemagglutination tests
Cross-reactivity
This Mab reacted only to Inaba-type cells; no agglutination was observed for Ogawa-type cells in either the slide agglutination or the microdilution agglutination tests. This Mab showed cross-reactivity to Brucella abortus
Proposed epitopes
N/A
Proposed Utility
This Mab showed strong agglutination with both live cells and heat-killed cells. This Mab will be a very useful tool for resolution of the antigenic structure of V. cholerae 01, especially the Inaba-specific determinant, as well as crossreactive structure to B. abortus. Moreover, highly Inabaspecific antibodies will be able to be used in the identification of Inaba serotypes instead of polyclonal Inaba-specific antisera
