PolysacDB 2498
From DrugPedia: A Wikipedia for Drug discovery
Revision as of 06:14, 8 April 2010
Carbohydrate Name
O-polysaccharide [Terminal tetrasaccharide]
Carbohydrate Class
O-polysaccharide [LPS]
Source Microbe
Vibrio cholerae O1 LPS serotype Inaba
Basic Structure
The O-antigen part consists of (1-->2)-α-linked 4-amino-4,6-dideoxy-D-mannose (perosamine) whose amino group is acylated with 3-deoxy-L-glycero-tetronic acid. The terminal sugar in the Inaba O-antigen has a hydroxyl at the 2' position
Proposed functions
V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodies
Glycoconjugates
Carrier Name
BSA
Conjugation Method
The purified carbohydrates were subjected to aminolysis to afford ethylenediamine derivatives, which were treated with squaric acid diethyl ester. The squaric acid monoesters, thus obtained, were treated with BSA to give neoglycoconjugates
Antibodies
Antsera
Antibody type and class
IgM and IgG3
Assay System
ELISA, invivo protection assay
Cross-reactivity
This antisera cross-reacted with V. cholerae Ogawa and Inaba LPS
Proposed epitopes
N/A
Proposed Utility
This antisera helped in the epitope studies of O1 LPS
