PolysacDB Pneumococcal vaccine OP 2467
From DrugPedia: A Wikipedia for Drug discovery
Revision as of 06:13, 8 April 2010
Carbohydrate Name
Pneumococcal vaccine OP
Carbohydrate Class
O-polysaccharide [LPS]
Source Microbe
Streptococcus pneumoniae
Basic Structure
This vaccine includes oligosaccharides of 6B, 14, 19F, 18C, 23F serotypes
Proposed functions
N/A
Antigenic Nature used to produce antibodies
Glycoconjugates
Carrier Name
Diphtheria toxin mutant (CRM197)
Conjugation Method
The oligosaccharide-CRM197 conjugates were synthesized by either first hydrolysing the native oligosaccharide [PS] to smaller fragments and then subjecting the fragments to limited periodate oxidation or by oxidizing the native PS and then hydrolyzing it into smaller fragments. The PS-CRM197 conjugates were synthesized by oxidizing native pneumococcal PS with sodium metaperiodate to introduce random aldehyde functional groups. Oxidized OS or PS fragments were coupled to CRM197 in the presence of sodium cyanoborohydride
Antibodies
Human Antisera
Antibody type and class
N/A
Assay System
ELISA, Opsonophagocytic killing assay
Cross-reactivity
The antisera reacted with 6B serotype, showed poor opsonophagocytic activity for 19F serotype. This antisera also cross-reacted with 6A serotype but did not react with 19A serotype
Proposed epitopes
N/A
Proposed Utility
The findings suggest that some conjugate vaccines may elicit cross-protection against related polysaccharides better than others
