PolysacDB O-polysaccharide 2309
From DrugPedia: A Wikipedia for Drug discovery
Revision as of 06:13, 8 April 2010
Carbohydrate Name
O-polysaccharide
Carbohydrate Class
O-polysaccharide [LPS]
Source Microbe
Shigella flexneri serotype 2a strain 2457T
Basic Structure
Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->
Proposed functions
LPS plays an important virulence role based on its antiphagocytic and endotoxic properties
Antigenic Nature used to produce antibodies
Whole cells
Carrier Name
nil
Conjugation Method
nil
Antibodies
Mab HYSF2a2
Antibody type and class
IgM
Assay System
ELISA and immunoblotting
Cross-reactivity
HySF2a2 showed the most restricted specificity, reacting only with S. flexneri 2a LPS
Proposed epitopes
This Mab is likely recognizing an epitope influenced by the presence of a single α-D-glycosyl residue on one of the rhamnose residues
Proposed Utility
This Mab may have implications for designing a Shigella vaccine that will be protective against related serotypes. This Mab may also be used to study the ultrastructural surface distribution of LPSs on the surface of the bacteria
