PolysacDB O-polysaccharide 2065

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Revision as of 06:11, 8 April 2010


Contents

Carbohydrate Name

O-polysaccharide

Carbohydrate Class

O-polysaccharide [LPS]

Source Microbe

Pseudomonas syringae serogroup O1 strain atrofaciens NCPPB 2612

Basic Structure

O-polysaccharides of the LPS consists of tetra- and tri-α-D-rhamnose repeats in the backbone [-3)D-Rha(α1->3)D-Rha(α1-->2)D-Rha(α1->2)D-Rha(α1] and [3)D-Rha(α1-->3)D-Rha(α1-->2)D-Rha(α1-]


Proposed functions

N/A

Antigenic Nature used to produce antibodies

Whole cells

Carrier Name

nil

Conjugation Method

nil

Antibodies

Mab Ps1C1

Antibody type and class

IgG3

Assay System

ELISA, agglutination and immunoblotting

Cross-reactivity

This Mab showed strong reactivity with OPS of chemotype 1C and weak reactivity with OPS of chemotype 1C-1A with a low content of 1A linear O repeats

Proposed epitopes

The epitope is related to the lateral D-GlcNAc residue and also one or more D-Rha residues of the backbone 1A and is more influenced by the protein-dependent conformation of the OPS chain

Proposed Utility

This Mab agglutinated heat-killed homologous bacterial cells

Weblink

PubMed Central

External Links