PolysacDB O-polysaccharide 2065
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Revision as of 06:11, 8 April 2010
Carbohydrate Name
O-polysaccharide
Carbohydrate Class
O-polysaccharide [LPS]
Source Microbe
Pseudomonas syringae serogroup O1 strain atrofaciens NCPPB 2612
Basic Structure
O-polysaccharides of the LPS consists of tetra- and tri-α-D-rhamnose repeats in the backbone [-3)D-Rha(α1->3)D-Rha(α1-->2)D-Rha(α1->2)D-Rha(α1] and [3)D-Rha(α1-->3)D-Rha(α1-->2)D-Rha(α1-]
Proposed functions
N/A
Antigenic Nature used to produce antibodies
Whole cells
Carrier Name
nil
Conjugation Method
nil
Antibodies
Mab Ps1C1
Antibody type and class
IgG3
Assay System
ELISA, agglutination and immunoblotting
Cross-reactivity
This Mab showed strong reactivity with OPS of chemotype 1C and weak reactivity with OPS of chemotype 1C-1A with a low content of 1A linear O repeats
Proposed epitopes
The epitope is related to the lateral D-GlcNAc residue and also one or more D-Rha residues of the backbone 1A and is more influenced by the protein-dependent conformation of the OPS chain
Proposed Utility
This Mab agglutinated heat-killed homologous bacterial cells