PolysacDB O-polysaccharide 2060
From DrugPedia: A Wikipedia for Drug discovery
Revision as of 06:11, 8 April 2010
Carbohydrate Name
O-polysaccharide
Carbohydrate Class
O-polysaccharide [LPS]
Source Microbe
Pseudomonas syringae IMV 120a pv. Phaseolicola
Basic Structure
O-polysaccharides of the LPS consists of tetra- and tri-α-D-rhamnose repeats in the backbone [-3)D-Rha(α1->3)D-Rha(α1-->2)D-Rha(α1->2)D-Rha(α1] and [3)D-Rha(α1-->3)D-Rha(α1-->2)D-Rha(α1-]
Proposed functions
N/A
Antigenic Nature used to produce antibodies
Whole cells
Carrier Name
nil
Conjugation Method
nil
Antibodies
Mab Ps1d
Antibody type and class
IgM
Assay System
Agglutination, Ouchterlony immunodiffusion, ELISA, SDS-PAGE, and Western immunoblotting
Cross-reactivity
"This Mab reacted with P. syringae strains with pathovars atrofaciens, phaseolicola and 17 (80%) P. syringae strains for which the pathovar(s) has not been identified"
Proposed epitopes
The epitope was confined to lateral immunodominant substituents of (α1-->3)D-Rha, (β1-->4)D-GlcNAc, and (α1-->4)D-Fuc, respectively, which are all bound to the backbone-located D-rhamnose
Proposed Utility
This Mab strongly agglutinated homologous bacterial cells and precipitated soluble antigens
