PolysacDB Glycopeptidolipid antigen 1667
From DrugPedia: A Wikipedia for Drug discovery
Revision as of 06:09, 8 April 2010
Carbohydrate Name
Glycopeptidolipid antigen
Carbohydrate Class
Miscellaneous
Source Microbe
Mycobacterium avium complex serovar 4
Basic Structure
This antigen is composed of a glycosylated lipopeptide core, fatty acyl-D-phenylalanine-D-allothreonine-D-alanine-L-alaninyl-O-(3,4-di-O-methyl-α-L-rhamnopyranoside), to which a haptenic oligosaccharide is linked at the threonine substituent
Proposed functions
This oligosaccharide unit is the source of the dominant immunogenicity of members of the M. avium complex, i.e., their characteristic agglutinability and type specificity
Antigenic Nature used to produce antibodies
serovar 4-specific neoglycoprotein, 4-0-methyl-a-L-rhamnopyranosyl(1--> 4)-2-0 - methyl - alpha - L fucopyranosyl oxynonanoyl-bovine serum albumin (BSA) (neoantigen 4) along with whole washed M. avium complex serovar 4 cells and corresponding serovar 4 glycopeptidolipid antigens
Carrier Name
nil
Conjugation Method
nil
Antibodies
Mab F85-3
Antibody type and class
IgG3
Assay System
ELISA, Inhibition assays, dot blot
Cross-reactivity
This antibody cross-reacted with whole cells of serovar 4 and acetylated-GPL-4
Proposed epitopes
4-O-methyl-α-L-rhamnopyranosyl-(1-->4)-2-O-methyl-α-L-fucopyranosyl residues seemed to be a part of the epitope
Proposed Utility
This Mab can be used for the identification and classification of M. avium isolates. This Mab can be applied directly to bacterial cultures, thus allowing easy, rapid identification of at least half of the M. avium isolates encountered in the clinical laboratory
