PolysacDB FVP2 polysaccharide 1452
From DrugPedia: A Wikipedia for Drug discovery
Revision as of 06:08, 8 April 2010
Carbohydrate Name
FVP2 polysaccharide
Carbohydrate Class
Miscellaneous
Source Microbe
Flammulina velutipes
Basic Structure
Consists of an α-(1-->4)-D-glucan, branching with a single α-D-glucan at the C-6 position to every seven residues along the main chain
Proposed functions
FVP2 can enhance the livability of primary culture of mouse hepatocytes in vitro and decrease the release of the alanine aminotransferase (ALT) as well as apoptosis of hepatocytes after the carbon tetrachloride (CCl4) intoxication. Furthermore, FVP2 has the capability of suppressing the growth of hepatoma 22 cells and sarcoma 180 cells (S180), extending the survival time of L615 leukemia mice, and promoting the lymphocyte transformation rate, natural killer cell activity, and the interleukin-2 production index in normal and tumor-bearing (S180) mice
Antigenic Nature used to produce antibodies
Glycoconjugates
Carrier Name
Bovine serum albumin
Conjugation Method
The FVP2 polysaccharide was conjugated to BSA using 1-cyano-4-dimethyl-aminopyridinium tetrafluoroborate (CDAP) treatment
Antibodies
Mab I6D
Antibody type and class
IgM
Assay System
Indirect ELISA, Double Immunodiffusion
Cross-reactivity
N/A
Proposed epitopes
N/A
Proposed Utility
This Mab provides a potentially useful tool for further study in the development of a novel and efficient immunoassay to detect FVP2 in biological specimens
