PolysacDB O-polysaccharide 1248
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Revision as of 06:03, 8 April 2010
Carbohydrate Name
O-polysaccharide
Carbohydrate Class
O-polysaccharide [LPS]
Source Microbe
Brucella melitensis 16M
Basic Structure
O-polysaccharide is a homopolymer of 4,6-dideoxy-4-formamidoct-D-mannopyranose residues
Proposed functions
N/A
Antigenic Nature used to produce antibodies
B.melitensis 16M formaldehyde-killed cells
Carrier Name
nil
Conjugation Method
nil
Antibodies
Mab BM3-8
Antibody type and class
IgG2a
Assay System
ELISA, immunodiffusion and competitive inhibition assay
Cross-reactivity
This antibody was specific to B. melitiensis and did not cross-reac with O-polysaccharide of B. abortus and Yersinia enterocolitica O:9 polysaccharide
Proposed epitopes
This Mab reacted only with M antigen. The crucial element of the M epitope is a disaccharide, α-D-Rha4NFo(1-->3)α-D Rha4NFo, that requires adjacent α1,2-linked residues for full activity. The common epitope of A and M antigens is a linear α1,2-linked tri or tetrasaccharide
Proposed Utility
This Mab can be used as a use as standard Brucella typing or detection reagents
