PolysacDB Smooth Lipopolysaccharide 1214

From DrugPedia: A Wikipedia for Drug discovery

Revision as of 06:03, 8 April 2010 (edit) Raghavagps (Talk | contribs) m (1 revision) ← Previous diff

Current revision (05:46, 9 September 2010) (edit) (undo) Raghavagps (Talk | contribs) m (1 revision)

---

Current revision

Contents 1 Carbohydrate Name 2 Carbohydrate Class 3 Source Microbe 4 Basic Structure 5 Proposed functions 6 Antigenic Nature used to produce antibodies 7 Carrier Name 8 Conjugation Method 9 Antibodies 10 Antibody type and class 11 Assay System 12 Cross-reactivity 13 Proposed epitopes 14 Proposed Utility 15 Weblink 16 External Links

[edit] Carbohydrate Name

Smooth Lipopolysaccharide

[edit] Carbohydrate Class

Lipopolysaccharide

[edit] Source Microbe

Rough Brucella melitensis strain B115

[edit] Basic Structure

The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M

[edit] Proposed functions

LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin

[edit] Antigenic Nature used to produce antibodies

Whole cells

[edit] Carrier Name

nil

[edit] Conjugation Method

nil

[edit] Antibodies

Mab 03D03

[edit] Antibody type and class

IgG3

[edit] Assay System

ELISA and immunoblotting

[edit] Cross-reactivity

This antibody was specific to a common epitope on A and M polysaccharides. But was more reactive towards M dominant strain B. melitensis 16M.This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS

[edit] Proposed epitopes

Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope

[edit] Proposed Utility

this mAb may be particularly useful for typingsmooth Brucella strains

[edit] Weblink

PubMed

[edit] External Links

• Link to PolysacDB database