PolysacDB Smooth Lipopolysaccharide 1205

From DrugPedia: A Wikipedia for Drug discovery

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Revision as of 06:03, 8 April 2010

Contents 1 Carbohydrate Name 2 Carbohydrate Class 3 Source Microbe 4 Basic Structure 5 Proposed functions 6 Antigenic Nature used to produce antibodies 7 Carrier Name 8 Conjugation Method 9 Antibodies 10 Antibody type and class 11 Assay System 12 Cross-reactivity 13 Proposed epitopes 14 Proposed Utility 15 Weblink 16 External Links

Carbohydrate Name

Smooth Lipopolysaccharide

Carbohydrate Class

Lipopolysaccharide

Source Microbe

Rough Brucella melitensis strain B115

Basic Structure

The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M

Proposed functions

LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin

Antigenic Nature used to produce antibodies

Whole cells

Carrier Name

nil

Conjugation Method

nil

Antibodies

Mab 07F09

Antibody type and class

IgG1

Assay System

ELISA and immunoblotting

Cross-reactivity

This antibody was specific to a common epitope on A and M polysaccharides. This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS

Proposed epitopes

Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope

Proposed Utility

this mAb may be particularly useful for typingsmooth Brucella strains

Weblink

PubMed

External Links

• Link to PolysacDB database