PolysacDB Lipopolysaccharide 1165
From DrugPedia: A Wikipedia for Drug discovery
Revision as of 06:03, 8 April 2010
Carbohydrate Name
Lipopolysaccharide
Carbohydrate Class
Lipopolysaccharide
Source Microbe
Bordetella pertusis 1414
Basic Structure
B. pertusis LPS is resolved electrophoretically into two bands, a slowly migrating band designated LOS A and a fast-migrating band designated LOS B. These two species differ in molecular weight as three N-acetyl amino sugars found on LOS A are not present on LOS B
Proposed functions
The LOS of B. pertussis exhibits biological activities similar to those of endotoxins from other gram-negative bacteria, including pyrogenicity, toxicity, induction of the Shwartzman reaction, induction of tumor necrosis factor production, and adjuvant properties
Antigenic Nature used to produce antibodies
B. pertusis LPS
Carrier Name
nil
Conjugation Method
nil
Antibodies
Mab D7
Antibody type and class
IgG3
Assay System
ELISA and Immunostaining
Cross-reactivity
did not cross-react with the B. pertusis variant strain A101
Proposed epitopes
The epitope recognized by D7 is affected by the addition of the O-side chain and that a portion of the distal trisaccharide could be involved in this epitope. This suggests therefore that this epitope may consist of the FucN(Ac)Me-->GlcN disaccharide
Proposed Utility
This Mab can be useful for rapid typing of Bordetella in clinical isolates and can be used to detect antigenic modifications in LPSs from variant B. pertassis strains, or from related Bordetella species (B. parapertassis, B. bronchiseptica
