PolysacDB Galactomannan 1132
From DrugPedia: A Wikipedia for Drug discovery
Revision as of 06:03, 8 April 2010
Carbohydrate Name
Galactomannan
Carbohydrate Class
Miscellaneous
Source Microbe
Aspergillus flavus EV 601
Basic Structure
Galactomannans are composed of an α-1,6-linked mannan backbone substituted with mannopyranose oligosaccharides and immunodominant terminal galactofuranose present either singly or linked to oligogalactosides
Proposed functions
The wide distribution and similar chemical structure of the galactomannans have been responsible for serologic cross-reactions
Antigenic Nature used to produce antibodies
Formalinized spores of A. fumigatus EV 601
Carrier Name
nil
Conjugation Method
nil
Antibodies
Mab EBA-4
Antibody type and class
IgM
Assay System
ELISA, Immunofluorescence and immunoblotting
Cross-reactivity
This Mab cross-reacted with the five Aspergillus species (A. fumigatus ATCC 1028, A. flavus ATCC 10124, A. niger ATCC 10549, A. versicolor ATCC 16845, and A. terreus CBS 106.25) but not with other medically important fungi, such as Sporothrix schenckii ATCC 14096 and Candida spp. (25 strains, including 10 ATCC strains, 2 CBS strains, and 13 clinical isolates, representing seven species, i.e., C. albicans, C. tropicalis, C. [Torulopsis] glabrata, C. pseudotropicalis, C. parapsilosis, C. guilliennondii, and C. krusei). They also failed to react with the 15 different bacterial species tested
Proposed epitopes
an epitope located on the β(1-->5) galactofuranose containing side chains of the galactomannan molecule
Proposed Utility
This rat Mab can be used against A. fumigatus galactomannan, already applied to the serological and immunohistological diagnosis of invasive aspergillosis
