PolysacDB High molecular weight polysaccharide 1108
From DrugPedia: A Wikipedia for Drug discovery
Revision as of 06:03, 8 April 2010
Carbohydrate Name
High molecular weight polysaccharide
Carbohydrate Class
Miscellaneous
Source Microbe
Actinobacillus pleuropneumoniae serotype 4 (field isolate F6)
Basic Structure
N/A
Proposed functions
N/A
Antigenic Nature used to produce antibodies
formalin-fixed A. pleuropneumoniae serotype 4 cells
Carrier Name
nil
Conjugation Method
nil
Antibodies
Mab 5H7
Antibody type and class
IgM
Assay System
Whole cell ELISA and slide agglutination tests
Cross-reactivity
This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. This Mab exhibited 75.8 and 27.3% reactivities with 29 serotype 4 and 12 serotype7 isolates, respectively, and partial binding to serotype 1 and nontypeable isolates
Proposed epitopes
N/A
Proposed Utility
This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7
