PolysacDB O-polysaccharide 1107
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Revision as of 06:03, 8 April 2010
Carbohydrate Name
O-polysaccharide
Carbohydrate Class
O-polysaccharide [LPS]
Source Microbe
Actinobacillus pleuropneumoniae serotype 4 (field isolate F6)
Basic Structure
O chains of LPS of serotypes 4 and 7 were structurally similar, being composed of repeating tetrasaccharide units with identical trisaccharide residues forming a common backbone
Proposed functions
LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus
Antigenic Nature used to produce antibodies
formalin-fixed A. pleuropneumoniae serotype 4 cells
Carrier Name
nil
Conjugation Method
nil
Antibodies
Mab 7C3
Antibody type and class
IgM
Assay System
Whole cell ELISA and slide agglutination tests
Cross-reactivity
This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. were reactive with 26 serotype 4 isolates,11 serotype 7 isolates, and 1 each of the serotype 1 and non-typeable isolates tested
Proposed epitopes
N/A
Proposed Utility
This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7
