Protein Refolding
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(New page: Recombinant DNA technology allows the expression of valuable heterologous proteins at high expression rates. Particularly in Escherichia coli (E. coli) overexpression of proteins often lea...)
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Revision as of 16:42, 31 August 2010
Recombinant DNA technology allows the expression of valuable heterologous proteins at high expression rates. Particularly in Escherichia coli (E. coli) overexpression of proteins often leads to aggregation and deposition in dense, insoluble particles within the host cell, so-called inclusion bodies (IB). Formation of inclusion bodies is heavily protein dependent, charge distribution and turn forming residues have a strong impact (1),
