CarboDB Glucurunoxylomannan 2640

From DrugPedia: A Wikipedia for Drug discovery

---

Revision as of 00:00, 15 January 2001

Contents 1 Carbohydrate Name 2 Carbohydrate Class 3 Source Microbe 4 Basic Structure 5 Proposed functions 6 Antigenic Nature used to produce antibodies 7 Carrier Name 8 Conjugation Method 9 Antibodies 10 Antibody type and class 11 Assay System 12 Cross-reactivity 13 Proposed epitopes 14 Proposed Utility 15 Weblink 16 External Links

Carbohydrate Name

Glucurunoxylomannan

Carbohydrate Class

Miscellaneous

Source Microbe

Cryptococcus neoformans strain 24067

Basic Structure

A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues

Proposed functions

Antiphagocytic ; an important virulence factor

Antigenic Nature used to produce antibodies

Glycoconjugates

Carrier Name

Diphtheria toxoid

Conjugation Method

5 mg of GXM (strain 24067) was activated with 5 mg of CDAP (1-cyano-4-dimethylaminopyridinium tetrafluoroborate) and conjugated to Diphtheria toxoid

Antibodies

Mab G15

Antibody type and class

IgM

Assay System

ELISA and immunofluorescence

Cross-reactivity

This antibody cross-reacted with Serotypes A and D in whole cell ELISA and immunofluorescence. Did not cross-react with Serotype A glucurunoxylomannan alone

Proposed epitopes

N/A

Proposed Utility

This MAb mediated complement deposition on solid phase GXM by ELISA. Administration of Mab G15 also prolonged the survival of C. neoformans

Weblink

PubMed

External Links

• Link to CarboDB database