CarboDB 2383
From DrugPedia: A Wikipedia for Drug discovery
Revision as of 00:00, 15 January 2001
Carbohydrate Name
Surface Polysaccharide Poly-N-Acetylglucosamine [PNAG]
Carbohydrate Class
Miscellaneous
Source Microbe
Staphylococcus aureus
Basic Structure
PNAG consists of a backbone of N-acetylglucosamine residues of variable lendth
Proposed functions
PNAG also referred to as the polysaccharide intercellular adhesin, has been shown to elicit opsonic antibodies when used as a vaccine in goats and rabbits. In addition, these polyclonal antibodies passively protect mice against S. aureus bacteremia and renal infection as well as against lethality following a high-dose infection. Animal antibodies to PNAG also mediate killing of S. epidermidis strains that express this antigen, and these strains constitute a significant proportion of clinical isolates
Antigenic Nature used to produce antibodies
Staphylococcus aureus infected patients
Carrier Name
nil
Conjugation Method
nil
Antibodies
Mab F598
Antibody type and class
IgG1
Assay System
enzyme-linked immunosorbent assays, complement deposition, immunofluorescence and opsonophagocytic assays
Cross-reactivity
This Mab was specfic to S. aureus and was found to be nonreactive against alginate from Pseudomonas aeruginosa strain PAO 581, the P. aeruginosa LPS O antigens from serogroups 02 and 06, and the PS/A antigen of Bacteroides fragilis
Proposed epitopes
This Mab bound best to nonacetylated or backbone epitopes on PNAG
Proposed Utility
This Mab had superior complement deposition and opsonophagocytic activity. This Mab also had optimal protective efficacy. This Mab could be immunotherapeutic agent for preventing or treating staphylococcal infections.
