CarboDB O-polysaccharide 2310

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Revision as of 00:00, 15 January 2001


Contents

Carbohydrate Name

O-polysaccharide

Carbohydrate Class

O-polysaccharide [LPS]

Source Microbe

Shigella flexneri serotype 2a strain 2457T

Basic Structure

Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->


Proposed functions

LPS plays an important virulence role based on its antiphagocytic and endotoxic properties

Antigenic Nature used to produce antibodies

Whole cells

Carrier Name

nil

Conjugation Method

nil

Antibodies

Mab HYSF2a3

Antibody type and class

IgM

Assay System

ELISA and immunoblotting

Cross-reactivity

HySF2a3 reacted only with 2a and 5a LPSs

Proposed epitopes

This Mab is likely recognizing an epitope influenced by the presence of a single α-D-glycosyl residue on one of the rhamnose residues

Proposed Utility

This Mab may have implications for designing a Shigella vaccine that will be protective against related serotypes. This Mab may also be used to study the ultrastructural surface distribution of LPSs on the surface of the bacteria

Weblink

PubMed Central

External Links