CarboDB Glycosphingolipid antigen 1535

From DrugPedia: A Wikipedia for Drug discovery

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Revision as of 00:00, 15 January 2001

Contents 1 Carbohydrate Name 2 Carbohydrate Class 3 Source Microbe 4 Basic Structure 5 Proposed functions 6 Antigenic Nature used to produce antibodies 7 Carrier Name 8 Conjugation Method 9 Antibodies 10 Antibody type and class 11 Assay System 12 Cross-reactivity 13 Proposed epitopes 14 Proposed Utility 15 Weblink 16 External Links

Carbohydrate Name

Glycosphingolipid antigen

Carbohydrate Class

Miscellaneous

Source Microbe

Leishmania amazonensis

Basic Structure

The structure of the glycosphingolipid is as follows : &alpha-Neup5Ac-(2-->3)-β-D-Galp-(1-->3)-β-D-GalpNAc-(1-->4)-β-D-Galp [branched to α-Neup5Ac-(2-->3)-] -(1-->4)-β-D-Glcp-(1-?)-CER-(?--/Ceramide/

Proposed functions

N/A

Antigenic Nature used to produce antibodies

L. amazonensis amastigotes

Carrier Name

nil

Conjugation Method

nil

Antibodies

Mab 1C3

Antibody type and class

N/A

Assay System

Indirect immunofluorescence, immunoblotting and immunoprecipitation

Cross-reactivity

This Mab reacted with amastigotes and cross-reacts with gp63 from L. amazonensis promastigotes

Proposed epitopes

ceramide-N-acetylneuraminic acid (GM1) and ceramide-N-tetrose-di-acetylneuraminic acid (GDla) residues seemed to be a part of the epitope

Proposed Utility

MAb 1C3 Fab fragments inhibited about 60% of the penetration of amastigotes into macrophages

Weblink

PubMed Central

External Links

• BCSDB Structural Detail

• Link to CarboDB database