CarboDB Lipopolysaccharide 1455
From DrugPedia: A Wikipedia for Drug discovery
Revision as of 00:00, 15 January 2001
Carbohydrate Name
Lipopolysaccharide
Carbohydrate Class
Lipopolysaccharide
Source Microbe
Haemophilus ducreyi
Basic Structure
It consist of an oligosaccharide core ketosidically linked via a 3-deoxy-D-manno-octulosonic acid (Kdo) residue to the lipid A region. The oligosaccharide core characteristic of these bacteria has been divided into two regions: (i) an inner core, consisting of three L-glycero-D-manno-heptose (Hep) residues and one phosphorylated Kdo residue, and (ii) an outer core, with one or more heterogeneous oligosaccharide branches containing mainly galactose (Gal) and glucose (Glc) residues
Proposed functions
LPS plays an important role in the infection process
Antigenic Nature used to produce antibodies
total membrane preparation of H. ducreyi
Carrier Name
nil
Conjugation Method
nil
Antibodies
Mab DP8
Antibody type and class
IgG1
Assay System
whole-cell enzyme immunoassay (Enzyme Immunoassay) and colony dot immunoblotting
Cross-reactivity
This Mab cross-reacted with 50 strains of H. ducreyi but not with any other bacteria tested
Proposed epitopes
phosphate groups seemed to be an important part of the epitope. The epitope consists of Glcβ1-->4Hepα1-->Kdo(P) that is linked via 1-->3 linkage to Hepα1-->2Hep
Proposed Utility
This Mab would help to detect the organism in environmental samples and clinical specimens
