Immunotation of Rv2753c (dihydrodipicolinate synthase)
From DrugPedia: A Wikipedia for Drug discovery
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<tr><td>sp|Q7Z7B0</td><td> 27.69</td><td> 44.62</td><td> 65</td><td> 2.7</td></tr></table> | <tr><td>sp|Q7Z7B0</td><td> 27.69</td><td> 44.62</td><td> 65</td><td> 2.7</td></tr></table> | ||
=Antigens= | =Antigens= | ||
| - | = | + | ==Epitopes== |
| - | =Epitopes= | + | |
===B-Cell Epitopes=== | ===B-Cell Epitopes=== | ||
====BCEpred==== | ====BCEpred==== | ||
| + | |||
Result Predicted by [http://www.imtech.res.in/raghava/bcepred/bcepred_submission.html bcelpep] | Result Predicted by [http://www.imtech.res.in/raghava/bcepred/bcepred_submission.html bcelpep] | ||
<br> | <br> | ||
| - | + | Result Predicited by [http://crdd.osdd.net/drugpedia/images/bcepred_rv2753c.pdf Predicited by ABCPred]]<br> | |
| - | SGDG<br> | + | <!--SGDG<br> |
VDQGCDG'''LV<br> | VDQGCDG'''LV<br> | ||
VSGTTGESPTTTDGEKIE'''<br> | VSGTTGESPTTTDGEKIE'''<br> | ||
'''PYYSKPPQ'''RG<br> | '''PYYSKPPQ'''RG<br> | ||
| - | '''VKDAKAD'''L | + | '''VKDAKAD'''L--!> |
<br><br><br> | <br><br><br> | ||
====ABCpred Results==== | ====ABCpred Results==== | ||
Revision as of 06:49, 5 January 2010
| Immunotation of Rv2753c (dihydrodipicolinate synthase)
| |
| Name | |
| Dihydrodipicolinate Synthase | |
| Identifiers | |
| Swiss Prot | |
| Genbank | |
| PDB | |
| Chemical data | |
| Formula | C28H37ClN4O6 [1] |
| Mol. wt. | 30,858 Da |
| Pharmacokinetic data | |
| Bioavailability | ? |
| Solubility | ? |
| Isoelectric-Point | 5.57 |
Contents |
General
dapA catalyzes the formation of dihydrodipicolinate from L-aspartate 4-semialdehyde and pyruvate.
L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2H2O.
This protein is eesential for optimal growth of M.tb and found in the cytoplasm as predicted by the WoLFPSORT and Subloc Server. HMMpfam found one domain PF00701 and has Helix Turn Helix secondary structure.
Protein Sequence
>Rv2753c, TB.seq 3066222:3067121 MW:30827 VTTVGFDVAARLGTLLTAMVTPFSGDGSLDTATAARLANHLVDQGCDGLVVSGTTGESPTTTDGEKIELLRAVLEAVGDR ARVIAGAGTYDTAHSIRLAKACAAEGAHGLLVVTPYYSKPPQRGLQAHFTAVADATELPMLLYDIPGRSAVPIEPDTIRA LASHPNIVGVKDAKADLHSGAQIMADTGLAYYSGDDALNLPWLAMGATGFISVIAHLAAGQLRELLSAFGSGDIATARKI NIAVAPLCNAMSRLGGVTLSKAGLRLQGIDVGDPRLPQVAATPEQIDALAADMRAASVLR
Human Homologue Blast Result
| subject ids | % identity | % positives | alignment length | evalue |
| sp|Q9BXD5 | 28 | 46.33 | 300 | 3.00E-023 |
| sp|Q86XE5 | 26.57 | 46.15 | 286 | 4.00E-022 |
| sp|Q9UG63 | 26.49 | 37.75 | 151 | 0.45 |
| sp|O14556 | 22.09 | 50 | 86 | 2 |
| sp|Q7Z7B0 | 27.69 | 44.62 | 65 | 2.7 |
Antigens
Epitopes
B-Cell Epitopes
BCEpred
Result Predicted by bcelpep
Result Predicited by Predicited by ABCPred]
Result Predicted by IEDB
|
PFSGDGSLDTA | |
|
GTTGESPTTTDGE | |
|
GTYDTAH | |
|
AEG | |
|
YSKPPQRGLQ | |
|
DATEL | |
|
RSAVPIEPDTIRA | |
|
KADLHSG | |
|
YSGDDALN | |
|
IATAR | |
|
GDPRLPQVAATPEQIDALAAD |
| Epitope | No of Alleles |
| VPIEPDTIR | 15 |
| TPFSGDGSL | 14 |
| TLSKAGLRL | 13 |
| IEPDTIRAL | 13 |
| GTYDTAHSI | 13 |
| DDALNLPWL | 11 |
| GFISVIAHL | 10 |
| GATGFISVI | 10 |
| ATPEQIDAL | 10 |
| YDTAHSIRL | 9 |
| GDIATARKI | 9 |
| AVLEAVGDR | 9 |
| ASHPNIVGV | 9 |
| QRGLQAHFT | 7 |
| GLQAHFTAV | 7 |
| AVGDRARVI | 7 |
| YSGDDALNL | 6 |
| VGFDVAARL | 6 |
| QLRELLSAF | 6 |
| TAVADATEL | 5 |
| TAARLANHL | 5 |
| LLRAVLEAV | 5 |
| GEKIELLRA | 5 |
| DTATAARLA | 5 |
| AAGQLRELL | 5 |
| TDGEKIELL | 4 |
| QAHFTAVAD | 4 |
| MSRLGGVTL | 4 |
| LDTATAARL | 4 |
| HPNIVGVKD | 4 |
| GVKDAKADL | 4 |
| DGEKIELLR | 4 |
| KIELLRAVL | 3 |
| HSIRLAKAC | 3 |
| GSGDIATAR | 3 |
| AHLAAGQLR | 3 |
| PTTTDGEKI | 2 |
| IAHLAAGQL | 2 |
| DLHSGAQIM | 2 |
Class-II Binder
Result predicted by NetMHCII
| Epitope | No of Alleles |
| TGFISVIAHLAAGQL | 6 |
| GFISVIAHLAAGQLR | 6 |
| PDTIRALASHPNIVG | 4 |
| GATGFISVIAHLAAG | 4 |
| ATGFISVIAHLAAGQ | 4 |
| TELPMLLYDIPGRSA | 3 |
| MGATGFISVIAHLAA | 3 |
| LVVTPYYSKPPQRGL | 3 |
| GQLRELLSAFGSGDI | 3 |
| FISVIAHLAAGQLRE | 3 |
| DTIRALASHPNIVGV | 3 |
| QLRELLSAFGSGDIA | 2 |
| PEQIDALAADMRAAS | 2 |
| NLPWLAMGATGFISV | 2 |
| LPWLAMGATGFISVI | 2 |
| LNLPWLAMGATGFIS | 2 |
| LLVVTPYYSKPPQRG | 2 |
| LGGVTLSKAGLRLQG | 2 |
| KAGLRLQGIDVGDPR | 2 |
| IAGAGTYDTAHSIRL | 2 |
| GLLVVTPYYSKPPQR | 2 |
| GGVTLSKAGLRLQGI | 2 |
| EPDTIRALASHPNIV | 2 |
| ELPMLLYDIPGRSAV | 2 |
| ATELPMLLYDIPGRS | 2 |
| ALNLPWLAMGATGFI | 2 |
| AGQLRELLSAFGSGD | 2 |
| AGLRLQGIDVGDPRL | 2 |
