Immunotation of Rv2753c (dihydrodipicolinate synthase)
From DrugPedia: A Wikipedia for Drug discovery
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VSGTTGESPTTTDGEKIE'''<br> | VSGTTGESPTTTDGEKIE'''<br> | ||
'''PYYSKPPQ'''RG<br> | '''PYYSKPPQ'''RG<br> | ||
| - | '''VKDAKAD'''L<br> | + | '''VKDAKAD'''L |
| - | + | <br><br><br> | |
| + | Result Predicited by [http://www.imtech.res.in/raghava/abcpred/ABC_submission.html ABCPred] | ||
<table border='1'><tr><td> | <table border='1'><tr><td> | ||
TPEQIDALAADMRAAS | TPEQIDALAADMRAAS | ||
Revision as of 09:32, 17 December 2009
| Immunotation of Rv2753c (dihydrodipicolinate synthase)
| |
| Name | |
| Dihydrodipicolinate Synthase | |
| Identifiers | |
| Swiss Prot | |
| Genbank | |
| PDB | |
| Chemical data | |
| Formula | ? |
| Mol. wt. | 30,858 Da |
| Pharmacokinetic data | |
| Bioavailability | ? |
| Solubility | ? |
| Isoelectric-Point | 5.57 |
Contents |
General
dapA catalyzes the formation of dihydrodipicolinate from L-aspartate 4-semialdehyde and pyruvate.
L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2H2O.
This protein is eesential for optimal growth of M.tb and found in the cytoplasm as predicted by the WoLFPSORT and Subloc Server. HMMpfam found one domain PF00701 and has Helix Turn Helix secondary structure.
Human Homologue Blast Result
| subject ids | % identity | % positives | alignment length | evalue |
| sp|Q9BXD5 | 28 | 46.33 | 300 | 3.00E-023 |
| sp|Q86XE5 | 26.57 | 46.15 | 286 | 4.00E-022 |
| sp|Q9UG63 | 26.49 | 37.75 | 151 | 0.45 |
| sp|O14556 | 22.09 | 50 | 86 | 2 |
| sp|Q7Z7B0 | 27.69 | 44.62 | 65 | 2.7 |
Antigens
Haptens
Epitopes
B-Cell Epitopes
Result Predicted by bcelpep
SGDG
VDQGCDGLV
VSGTTGESPTTTDGEKIE
PYYSKPPQRG
VKDAKADL
Result Predicited by ABCPred
|
TPEQIDALAADMRAAS |
|
PGRSAVPIEPDTIRAL |
|
GTTGESPTTTDGEKIE |
|
QRGLQAHFTAVADATE |
|
AGAGTYDTAHSIRLAK |
|
DGLVVSGTTGESPTTT |
|
GIDVGDPRLPQVAATP |
|
QIMADTGLAYYSGDDA |
|
GTLLTAMVTPFSGDGS |
|
TPYYSKPPQRGLQAHF |
|
SGDIATARKINIAVAP |
|
DVAARLGTLLTAMVTP |
|
VGVKDAKADLHSGAQI |
|
MLLYDIPGRSAVPIEP |
|
PWLAMGATGFISVIAH |
MHC class I restricted T cell epitopes
CTL Epitopes
MHC Binder
Class-I Binder
Result Predicted by nHLApred
| Epitope | No of Alleles |
| VPIEPDTIR | 15 |
| TPFSGDGSL | 14 |
| TLSKAGLRL | 13 |
| IEPDTIRAL | 13 |
| GTYDTAHSI | 13 |
| DDALNLPWL | 11 |
| GFISVIAHL | 10 |
| GATGFISVI | 10 |
| ATPEQIDAL | 10 |
| YDTAHSIRL | 9 |
| GDIATARKI | 9 |
| AVLEAVGDR | 9 |
| ASHPNIVGV | 9 |
| QRGLQAHFT | 7 |
| GLQAHFTAV | 7 |
| AVGDRARVI | 7 |
| YSGDDALNL | 6 |
| VGFDVAARL | 6 |
| QLRELLSAF | 6 |
| TAVADATEL | 5 |
| TAARLANHL | 5 |
| LLRAVLEAV | 5 |
| GEKIELLRA | 5 |
| DTATAARLA | 5 |
| AAGQLRELL | 5 |
| TDGEKIELL | 4 |
| QAHFTAVAD | 4 |
| MSRLGGVTL | 4 |
| LDTATAARL | 4 |
| HPNIVGVKD | 4 |
| GVKDAKADL | 4 |
| DGEKIELLR | 4 |
| KIELLRAVL | 3 |
| HSIRLAKAC | 3 |
| GSGDIATAR | 3 |
| AHLAAGQLR | 3 |
| PTTTDGEKI | 2 |
| IAHLAAGQL | 2 |
| DLHSGAQIM | 2 |
Class-II Binder
Result predicted by NetMHCII
| Epitope | No of Alleles |
| TGFISVIAHLAAGQL | 6 |
| GFISVIAHLAAGQLR | 6 |
| PDTIRALASHPNIVG | 4 |
| GATGFISVIAHLAAG | 4 |
| ATGFISVIAHLAAGQ | 4 |
| TELPMLLYDIPGRSA | 3 |
| MGATGFISVIAHLAA | 3 |
| LVVTPYYSKPPQRGL | 3 |
| GQLRELLSAFGSGDI | 3 |
| FISVIAHLAAGQLRE | 3 |
| DTIRALASHPNIVGV | 3 |
| QLRELLSAFGSGDIA | 2 |
| PEQIDALAADMRAAS | 2 |
| NLPWLAMGATGFISV | 2 |
| LPWLAMGATGFISVI | 2 |
| LNLPWLAMGATGFIS | 2 |
| LLVVTPYYSKPPQRG | 2 |
| LGGVTLSKAGLRLQG | 2 |
| KAGLRLQGIDVGDPR | 2 |
| IAGAGTYDTAHSIRL | 2 |
| GLLVVTPYYSKPPQR | 2 |
| GGVTLSKAGLRLQGI | 2 |
| EPDTIRALASHPNIV | 2 |
| ELPMLLYDIPGRSAV | 2 |
| ATELPMLLYDIPGRS | 2 |
| ALNLPWLAMGATGFI | 2 |
| AGQLRELLSAFGSGD | 2 |
| AGLRLQGIDVGDPRL | 2 |
