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Revision as of 07:52, 5 January 2010

Immunotation of Rv2753c (dihydrodipicolinate synthase)
Name
Dihydrodipicolinate Synthase
Identifiers
Swiss Prot	P63945
Genbank	888289
PDB	1XXX
Chemical data
Formula	C28H37ClN4O6 [1]
Mol. wt.	30,858 Da
Pharmacokinetic data
Bioavailability	?
Solubility	?
Isoelectric-Point	5.57

1 General
- 1.1 Protein Sequence
2 Human Homologue Blast Result
3 Antigens
- 3.1 Epitopes
  - 3.1.1 B-Cell Epitopes
    - 3.1.1.1 BCEpred
    - 3.1.1.2 ABCpred Results
  - 3.1.2 IEBB Analysis
4 MHC Class-I Binder
- 4.1 MHC Class-II Binder
5 Alergen Protein
6 Antibacterial
7 Bacterial Toxin
8 External Links

General

dapA catalyzes the formation of dihydrodipicolinate from L-aspartate 4-semialdehyde and pyruvate.

L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2H₂O.

This protein is eesential for optimal growth of M.tb and found in the cytoplasm as predicted by the WoLFPSORT and Subloc Server. HMMpfam found one domain PF00701 and has Helix Turn Helix secondary structure.

Protein Sequence

>Rv2753c, TB.seq 3066222:3067121 MW:30827 VTTVGFDVAARLGTLLTAMVTPFSGDGSLDTATAARLANHLVDQGCDGLVVSGTTGESPTTTDGEKIELLRAVLEAVGDR ARVIAGAGTYDTAHSIRLAKACAAEGAHGLLVVTPYYSKPPQRGLQAHFTAVADATELPMLLYDIPGRSAVPIEPDTIRA LASHPNIVGVKDAKADLHSGAQIMADTGLAYYSGDDALNLPWLAMGATGFISVIAHLAAGQLRELLSAFGSGDIATARKI NIAVAPLCNAMSRLGGVTLSKAGLRLQGIDVGDPRLPQVAATPEQIDALAADMRAASVLR

Human Homologue Blast Result

subject ids	% identity	% positives	alignment length	evalue
sp\|Q9BXD5	28	46.33	300	3.00E-023
sp\|Q86XE5	26.57	46.15	286	4.00E-022
sp\|Q9UG63	26.49	37.75	151	0.45
sp\|O14556	22.09	50	86	2
sp\|Q7Z7B0	27.69	44.62	65	2.7

Antigens

Epitopes

B-Cell Epitopes

BCEpred

Link to bcelpep
Result Predicited by Predicited by ABCPred

ABCpred Results

Link to ABCpred
Result Predicited by Predicited by ABCPred

IEBB Analysis

Result Predicted by IEDB
Result Predicited by Predicited by IEDB

MHC Class-I Binder

Result Predicited by Predicited by nHLApred
Result Predicted by nHLApred

Epitope	No of Alleles
VPIEPDTIR	15
TPFSGDGSL	14
TLSKAGLRL	13
IEPDTIRAL	13
GTYDTAHSI	13
DDALNLPWL	11
GFISVIAHL	10
GATGFISVI	10
ATPEQIDAL	10
YDTAHSIRL	9
GDIATARKI	9
AVLEAVGDR	9
ASHPNIVGV	9
QRGLQAHFT	7
GLQAHFTAV	7
AVGDRARVI	7
YSGDDALNL	6
VGFDVAARL	6
QLRELLSAF	6
TAVADATEL	5
TAARLANHL	5
LLRAVLEAV	5
GEKIELLRA	5
DTATAARLA	5
AAGQLRELL	5
TDGEKIELL	4
QAHFTAVAD	4
MSRLGGVTL	4
LDTATAARL	4
HPNIVGVKD	4
GVKDAKADL	4
DGEKIELLR	4
KIELLRAVL	3
HSIRLAKAC	3
GSGDIATAR	3
AHLAAGQLR	3
PTTTDGEKI	2
IAHLAAGQL	2
DLHSGAQIM	2

MHC Class-II Binder

Result predicted by NetMHCII

Epitope	No of Alleles
TGFISVIAHLAAGQL	6
GFISVIAHLAAGQLR	6
PDTIRALASHPNIVG	4
GATGFISVIAHLAAG	4
ATGFISVIAHLAAGQ	4
TELPMLLYDIPGRSA	3
MGATGFISVIAHLAA	3
LVVTPYYSKPPQRGL	3
GQLRELLSAFGSGDI	3
FISVIAHLAAGQLRE	3
DTIRALASHPNIVGV	3
QLRELLSAFGSGDIA	2
PEQIDALAADMRAAS	2
NLPWLAMGATGFISV	2
LPWLAMGATGFISVI	2
LNLPWLAMGATGFIS	2
LLVVTPYYSKPPQRG	2
LGGVTLSKAGLRLQG	2
KAGLRLQGIDVGDPR	2
IAGAGTYDTAHSIRL	2
GLLVVTPYYSKPPQR	2
GGVTLSKAGLRLQGI	2
EPDTIRALASHPNIV	2
ELPMLLYDIPGRSAV	2
ATELPMLLYDIPGRS	2
ALNLPWLAMGATGFI	2
AGQLRELLSAFGSGD	2
AGLRLQGIDVGDPRL	2

Result Predicited by Propred

Epitope	No of Alleles
IRLAKACAA	51
INIAVAPLC	51
VVSGTTGES	48
LLVVTPYYS	48
IRALASHPN	46
LTAMVTPFS	44
LQAHFTAVA	43
LGTLLTAMV	43
LLTAMVTPF	42
VAPLCNAMS	41
IATARKINI	40
LRELLSAFG	38
LLYDIPGRS	38
LVVSGTTGE	37
VIAHLAAGQ	36
MLLYDIPGR	36
LRLQGIDVG	35
ISVIAHLAA	34
LQGIDVGDP	33
FISVIAHLA	33
WLAMGATGF	32
LLRAVLEAV	30
IELLRAVLE	30
YYSKPPQRG	25
IAVAPLCNA	24
LAADMRAAS	22
IVGVKDAKA	22
LCNAMSRLG	21
LANHLVDQG	21
LAMGATGFI	21
FGSGDIATA	20
YSGDDALNL	19
VGFDVAARL	18
LRAVLEAVG	17
LGGVTLSKA	17
IEPDTIRAL	17
LVVTPYYSK	16
LSKAGLRLQ	16
LHSGAQIMA	16
IMADTGLAY	16
IAHLAAGQL	16
VGVKDAKAD	15
MVTPFSGDG	15
LASHPNIVG	15
FSGDGSLDT	14
LNLPWLAMG	13
VGDRARVIA	12
IAGAGTYDT	11
FDVAARLGT	11
IPGRSAVPI	10
VTPYYSKPP	9
MSRLGGVTL	9
LSAFGSGDI	9
YDTAHSIRL	8
VTLSKAGLR	6
MGATGFISV	5
LYDIPGRSA	5
LVDQGCDGL	5
LAYYSGDDA	5
VAARLGTLL	4
LDTATAARL	4
VTPFSGDGS	3
VPIEPDTIR	3
LLSAFGSGD	3
LAKACAAEG	3
YSKPPQRGL	2
YDIPGRSAV	2
VVTPYYSKP	2
LPQVAATPE	2
LEAVGDRAR	2
LAAGQLREL	2

Alergen Protein

Non Allergen Predicted by AlgPred Server

Antibacterial

Bacterial Toxin

External Links

@@ Line 57: / Line 57: @@
 Result Predicted by [http://tools.immuneepitope.org/tools/bcell/iedb_input IEDB]<br>
 Result Predicited by  [http://crdd.osdd.net/drugpedia/images/IEDB_rv2753c.pdf Predicited by IEDB]<br>
-===MHC class I restricted T cell epitopes===
+=MHC Class-I Binder=
-=MHC Binder=
-===Class-I Binder===
 Result Predicited by  [http://crdd.osdd.net/drugpedia/images/Nhlapred_rv2753c.pdf Predicited by nHLApred]<br>
 Result Predicted by
@@ Line 148: / Line 145: @@
 </table>
-===Class-II Binder===
+===MHC Class-II Binder===
 Result predicted by [http://www.cbs.dtu.dk/services/NetMHCII/ NetMHCII]
 <table border='1'><tr><td>

Immunotation of Rv2753c (dihydrodipicolinate synthase)