Indipedia: India's Wikipedia at OSDD:General disclaimer

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-	Where are we standing?????????????????????	+	General disclaimer - Use Indipedia at your own risk!
-	After 126 years, the etiological agent of deadly tuberculosis (http://en.wikipedia.org/wiki/Tuberculosis) has been discovered by Robert Koch, we have only one vaccine and few countable antibiotics against tuberculosis. Is this justifiable?	+	PLEASE READ THE STATEMENT BELOW CAREFULLY BEFORE LEAVING THIS PAGE
-	Recent estimates of Tuberculosis disease is approximately 1.86 billion people with 16.2 million cases of active diseases. (Dye etal.,1999). It’s a fact and I also support that not a single treatment but a combinatorial approach is required against this culprit. We are living in the age of biotechnology and Nanotechnology so we should look for molecular medicine which may be some involve synthetic  molecules mimicking that in system approaches.  Through this article I have tried to make people think about the computers, biomolecules and some naturally occurring compounds as a combinatorial approach to fight the evil like tuberculosis.	+
-	In my this report I am suggesting one of my idea although its very older approach which has been discovered in 20th century that is Antisense DNA technology (http://en.wikipedia.org/wiki/Antisense_therapy). I have targeted the dna N gene (Gene ID 887092) (http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=57116681 from 2052 to 3260) of Mycobacterium tuberculosis H37Rv strain which is a DNA Polymerase III and is a complex, multi-chain enzyme responsible for most of the replicative process in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity.	+
-	A brief description of the method is as follows:	+	INDIPEDIA MAKES NO GUARANTEE OF VALIDITY
		+	It is an online open-content collection of source texts, that is, a voluntary association of individuals and groups who are developing a common resource for drug discovery. Its structure allows any individual with an Internet connection and World Wide Web browser to alter the content found here. Therefore, please be advised that nothing found here has necessarily been reviewed by professionals who are knowledgeable in the particular areas of expertise necessary to ensure the correctness of the texts.
-	•The sequence of the dna N gene has been analyzed for GC rich region which is from 2152 to 2172 of coding region (Total length of dna N gene in the genome of Mycobacterium 2052 to 3260).	+	That's not to say that you won't find much valuable and accurate information at Wikisource, however please be advised that Indipedia CANNOT guarantee, in any way whatsoever, the validity of the documents found here. It may recently have been changed or vandalized.
		+	None of the authors, contributors, sponsors, administrators, sysops, or anyone else connected with Wikisource in any way whatsoever can be responsible for the appearance of any inaccurate or libelous information or your use of the information contained in or linked from these web pages.
-	•The sequence was aligned with both the human genome (host of Mycobacterium tuberculosis) and Mycobacterium genome itself using the BLAST tool.	+	Please make sure that you understand that most of the information provided here is in the public domain. You are free to reproduce it freely and gratuitously just as we are doing. All other material is being provided free and gratuitously, and that no kind of agreement or contract is created between you and the owners or users of this site, the owners of the servers upon which it is housed, the individual Wikisource contributors, any project administrators, sysops or anyone else who is in any way connected with this project or sister projects subject to your claims against them directly. You are being granted a limited license to copy anything from this site; it does not create or imply any contractual or extracontractual liability on the part of Wikisource any of its agents, members, organizers or other users.
-	•It was found that there was no significant homology between the genome of human and the oligonucleotide we are going to use for Antisense against dna N gene. This shows that the oligo would act specifically against Mycobacterium.	+	Please note that the information found here may be in violation of the laws of the country or jurisdiction from where you are viewing this information. Wikisource does not encourage the violation of any laws, but as this information is stored on a server at Institute of microbial technology, Chandigarh, India it is being maintained. The laws in your country may not recognize as broad a protection of free speech as the laws of Government of India or the principles under the UN Charter, and as such, Wikisource cannot be responsible for any potential violations of such laws should you link to this domain or use any of the information contained herein in anyway whatsoever.
-	•It was also observed that the oligo has homology with other gene of Mycobacterium tuberculosis H37Rv strain.	+	Indipedia is not uniformly peer reviewed; while readers may correct errors or remove erroneous suggestions they have no legal duty to do so and thus all information read here is without any implied warranty of fitness for any purpose or use whatsoever.
-	•Then an expression vector (eg:  pCS 105 vector modified by Duve Turner’s) has been designed that will produce the antisense against the transcribed portion of dna N gene.	+	No consequential damages can be sought against Indipedia, as it is a voluntary association of individuals developed freely to create various open source online educational, cultural and informational resources. This information is being given to you gratuitously and there is no agreement or understanding between you and Indipedia regarding your use or modification of this information beyond the GNU Free Documentation License; neither is anyone at Wikisource responsible should someone change, edit, modify or remove any information that you may post on Wikisource or any of its associated projects.
-	•This vector has been transfected into the cell.	+	Thank you for spending the time to read this page, and please enjoy your experience at Wikisource.
-		+
-	•The vector would transcribe the antisense and this antisense result in DNA/RNA hybrid and RNase H degrade that hybrid resulting in no translation and killing of our target.	+
-	This report is just an idea how we can utilize the molecular medicine and the drugs which are available to treat nasty tuberculosis. Although people have tried such approaches so I think computational biology alongwith our knowledge of disease can help in knocking out deadly pathogens.	+
-		+
-	Courtesy: http://venturebeat.com/wp-content/uploads/2007/11/ .This picture is showing how  antisense therapy woks to inhibit expression of particular gene.	+
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-	References:	+
-		+
-	Dye,C., Scheele,S., Dolin,P., Pathania,V., and Raviglione,R.C. (1999). Global burden of tuberculosis—estimated incidence, prevalence, and mortality by country. J. Am. Med. Assn. 282,677–686	+

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Current revision

General disclaimer - Use Indipedia at your own risk!

PLEASE READ THE STATEMENT BELOW CAREFULLY BEFORE LEAVING THIS PAGE

INDIPEDIA MAKES NO GUARANTEE OF VALIDITY It is an online open-content collection of source texts, that is, a voluntary association of individuals and groups who are developing a common resource for drug discovery. Its structure allows any individual with an Internet connection and World Wide Web browser to alter the content found here. Therefore, please be advised that nothing found here has necessarily been reviewed by professionals who are knowledgeable in the particular areas of expertise necessary to ensure the correctness of the texts.

That's not to say that you won't find much valuable and accurate information at Wikisource, however please be advised that Indipedia CANNOT guarantee, in any way whatsoever, the validity of the documents found here. It may recently have been changed or vandalized. None of the authors, contributors, sponsors, administrators, sysops, or anyone else connected with Wikisource in any way whatsoever can be responsible for the appearance of any inaccurate or libelous information or your use of the information contained in or linked from these web pages.

Please make sure that you understand that most of the information provided here is in the public domain. You are free to reproduce it freely and gratuitously just as we are doing. All other material is being provided free and gratuitously, and that no kind of agreement or contract is created between you and the owners or users of this site, the owners of the servers upon which it is housed, the individual Wikisource contributors, any project administrators, sysops or anyone else who is in any way connected with this project or sister projects subject to your claims against them directly. You are being granted a limited license to copy anything from this site; it does not create or imply any contractual or extracontractual liability on the part of Wikisource any of its agents, members, organizers or other users.

Please note that the information found here may be in violation of the laws of the country or jurisdiction from where you are viewing this information. Wikisource does not encourage the violation of any laws, but as this information is stored on a server at Institute of microbial technology, Chandigarh, India it is being maintained. The laws in your country may not recognize as broad a protection of free speech as the laws of Government of India or the principles under the UN Charter, and as such, Wikisource cannot be responsible for any potential violations of such laws should you link to this domain or use any of the information contained herein in anyway whatsoever.

Indipedia is not uniformly peer reviewed; while readers may correct errors or remove erroneous suggestions they have no legal duty to do so and thus all information read here is without any implied warranty of fitness for any purpose or use whatsoever.

No consequential damages can be sought against Indipedia, as it is a voluntary association of individuals developed freely to create various open source online educational, cultural and informational resources. This information is being given to you gratuitously and there is no agreement or understanding between you and Indipedia regarding your use or modification of this information beyond the GNU Free Documentation License; neither is anyone at Wikisource responsible should someone change, edit, modify or remove any information that you may post on Wikisource or any of its associated projects.

Thank you for spending the time to read this page, and please enjoy your experience at Wikisource.