PolysacDB Lipopolysaccharide 2351

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Contents 1 Carbohydrate Name 2 Carbohydrate Class 3 Source Microbe 4 Basic Structure 5 Proposed functions 6 Antigenic Nature used to produce antibodies 7 Carrier Name 8 Conjugation Method 9 Antibodies 10 Antibody type and class 11 Assay System 12 Cross-reactivity 13 Proposed epitopes 14 Proposed Utility 15 Weblink 16 External Links

[edit] Carbohydrate Name

Lipopolysaccharide

[edit] Carbohydrate Class

Lipopolysaccharide

[edit] Source Microbe

Sinorhizobium meliloti strain NRG34

[edit] Basic Structure

There are two forms of LPS: rough LPS (R-LPS), which consists of a lipid A membrane anchor and conserved core oligosaccharides, and smooth LPS (S-LPS), which includes the O-antigen (or O-polysaccharide)

[edit] Proposed functions

LPS is an important surface antigen

[edit] Antigenic Nature used to produce antibodies

Whole cells

[edit] Carrier Name

nil

[edit] Conjugation Method

nil

[edit] Antibodies

Mab 10

[edit] Antibody type and class

IgG3

[edit] Assay System

ELISA and immunoblotting

[edit] Cross-reactivity

This Mab was highly cross-reactive recognizing most number of Shinorhizobium species. Strain-cross-reactive MAb 10 appeared to recognize several forms of R-LPS, as shown by the disperse signal in the R-LPS banding region, as well as the S-LPS from the same strains

[edit] Proposed epitopes

N/A

[edit] Proposed Utility

The use of MAbs to study specific cell surface components from the bacteroids of Rhizobium leguminosarum have yielded interesting information about morphogenesis in the pea symbiont, including the temporal expression of specific epitopes during nodule development

[edit] Weblink

PubMed

[edit] External Links

• Link to PolysacDB database