PolysacDB Capsular polysaccharide 1822

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Contents 1 Carbohydrate Name 2 Carbohydrate Class 3 Source Microbe 4 Basic Structure 5 Proposed functions 6 Antigenic Nature used to produce antibodies 7 Carrier Name 8 Conjugation Method 9 Antibodies 10 Antibody type and class 11 Assay System 12 Cross-reactivity 13 Proposed epitopes 14 Proposed Utility 15 Weblink 16 External Links

[edit] Carbohydrate Name

Capsular polysaccharide

[edit] Carbohydrate Class

Capsular polysaccharide

[edit] Source Microbe

Neisseria meningitidis group C

[edit] Basic Structure

It consists of a homopolymer of α(2-->8)-linked sialic acid residues

[edit] Proposed functions

It is an important virulence factor

[edit] Antigenic Nature used to produce antibodies

Glycoconjugates

[edit] Carrier Name

Tetanus toxoid

[edit] Conjugation Method

The oligosaccharide was activated using NaIO4 at 4C for 60 min and combined with monomeric tetanus toxoid in sodium phosphate buffer containing NaBH3CN and heated at 37C for 5 days

[edit] Antibodies

Mab C2/706.12

[edit] Antibody type and class

IgG1

[edit] Assay System

ELISA

[edit] Cross-reactivity

This Mab cross-reacted with the non-O-acetylated variant of capsular polysaccharide and also cross-reacted with E. coli K92 capsular polysaccharide

[edit] Proposed epitopes

α(2-->9)-linked sialic acid backbone seemed to be the part of the epitope

[edit] Proposed Utility

This Mab can be used for studying the capsular polysaccharide immunogenicity

[edit] Weblink

PubMed Central

[edit] External Links

• BCSDB Structural Detail

• Link to PolysacDB database