CarboDB Lipopolysaccharide 2351
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[edit] Carbohydrate Name
Lipopolysaccharide
[edit] Carbohydrate Class
Lipopolysaccharide
[edit] Source Microbe
Sinorhizobium meliloti strain NRG34
[edit] Basic Structure
There are two forms of LPS: rough LPS (R-LPS), which consists of a lipid A membrane anchor and conserved core oligosaccharides, and smooth LPS (S-LPS), which includes the O-antigen (or O-polysaccharide)
[edit] Proposed functions
LPS is an important surface antigen
[edit] Antigenic Nature used to produce antibodies
Whole cells
[edit] Carrier Name
nil
[edit] Conjugation Method
nil
[edit] Antibodies
Mab 10
[edit] Antibody type and class
IgG3
[edit] Assay System
ELISA and immunoblotting
[edit] Cross-reactivity
This Mab was highly cross-reactive recognizing most number of Shinorhizobium species. Strain-cross-reactive MAb 10 appeared to recognize several forms of R-LPS, as shown by the disperse signal in the R-LPS banding region, as well as the S-LPS from the same strains
[edit] Proposed epitopes
N/A
[edit] Proposed Utility
The use of MAbs to study specific cell surface components from the bacteroids of Rhizobium leguminosarum have yielded interesting information about morphogenesis in the pea symbiont, including the temporal expression of specific epitopes during nodule development
