CarboDB Lipopolysaccharide 1571
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[edit] Carbohydrate Name
Lipopolysaccharide
[edit] Carbohydrate Class
Lipopolysaccharide
[edit] Source Microbe
Leptospira interrogans serovar pomona
[edit] Basic Structure
The oligosaccharide contained rhamnose, ribose, glucose, and glucosamine
[edit] Proposed functions
N/A
[edit] Antigenic Nature used to produce antibodies
Glycoconjugates
[edit] Carrier Name
Diphtheria toxoid
[edit] Conjugation Method
The oligosaccharide was modified as follows : it was dissolved in N-ethylmorpholine-water-12 N HCl (2.5:6.5:1 [vol/vol/vol], pH 8.5) in concentrations of approximately 24 umol in 250ul. 125umol of N-succinimidyl S-acetylmercaptoacetate in 250ul of N,N dimethylacetamide was added to the oligosaccharide. This solution was added to modified diphtheria toxoid
[edit] Antibodies
Polysera
[edit] Antibody type and class
IgG and IgM
[edit] Assay System
Enzyme Immunoassay, immunoblotting and dot blot
[edit] Cross-reactivity
This antisera cross-reacted with LPS from some other leptospiral serovars (serovars hardjobovis, ballum, copenhageni, canicola, grippotyphosa, and batavia) and LPS from other bacteria (Salmonella typhimurium and the Re mutant of Shigella flexneri) and did not react with serovars australis, grippotyphosa, ballum, or tarassovi
[edit] Proposed epitopes
N/A
[edit] Proposed Utility
This antiserum induced a high CL response in mouse macrophage monolayers
