From DrugPedia: A Wikipedia for Drug discovery

Revision as of 11:55, 5 February 2010

General

Hexameric DnaB helicase unwinds the DNA double helix during replication of genetic material in bacteria. DnaB is an essential bacterial protein; therefore, it is an important potential target for antibacterial drug discovery.A crystal structure of the N-terminal region of DnaB from the pathogen Mycobacterium tuberculosis (MtDnaBn), determined at 2.0 A resolution have been reported. This structure provides atomic resolution details of formation of the hexameric ring of DnaB by two distinct interfaces. An extensive hydrophobic interface stabilizes a dimer of MtDnaBn by forming a four-helix bundle. The other, less extensive, interface is formed between the dimers, connecting three of them into a hexameric ring. On the basis of crystal packing interactions between MtDnaBn rings,a model of a helicase-primase complex that explains previously observed effects of DnaB mutations on DNA priming have been suggested. A characterization of the physical interactions between M. tuberculosis DnaA and DnaB using both in vivo methods, such as bacterial two hybrid assays, and in vitro techniques, such as surface plasmon resonance (SPR) and Pull down/Western blotting. The full length N-terminus (1206 residues) of DnaB was found to interact with DnaA, while the shorter N terminal domain of DnaB (1125 residues), which lacked the linker region, did not.

Protein Sequence

>Rv0058, TB.seq 60394:63015 MW:96919 MAVVDDLAPGMDSSPPSEDYGRQPPQDLAAEQSVLGGMLLSKDAIADVLERLRPGDFYRPAHQNVYDAILDLYGRGEPAD AVTVAAELDRRGLLRRIGGAPYLHTLISTVPTAANAGYYASIVAEKALLRRLVEAGTRVVQYGYAGAEGADVAEVVDRAQ AEIYDVADRRLSEDFVALEDLLQPTMDEIDAIASSGGLARGVATGFTELDEVTNGLHPGQMVIVAARPGVGKSTLGLDFM RSCSIRHRMASVIFSLEMSKSEIVMRLLSAEAKIKLSDMRSGRMSDDDWTRLARRMSEISEAPLFIDDSPNLTMMEIRAK ARRLRQKANLKLIVVDYLQLMTSGKKYESRQVEVSEFSRHLKLLAKELEVPVVAISQLNRGPEQRTDKKPMLADLRESGC LTASTRILRADTGAEVAFGELMRSGERPMVWSLDERLRMVARPMINVFPSGRKEVFRLRLASGREVEATGSHPFMKFEGW TPLAQLKVGDRIAAPRRVPEPIDTQRMPESELISLARMIGDGSCLKNQPIRYEPVDEANLAAVTVSAAHSDRAAIRDDYL AARVPSLRPARQRLPRGRCTPIAAWLAGLGLFTKRSHEKCVPEAVFRAPNDQVALFLRHLWSAGGSVRWDPTNGQGRVYY GSTSRRLIDDVAQLLLRVGIFSWITHAPKLGGHDSWRLHIHGAKDQVRFLRHVGVHGAEAVAAQEMLRQLKGPVRNPNLD SAPKKVWAQVRNRLSAKQMMDIQLHEPTMWKHSPSRSRPHRAEARIEDRAIHELARGDAYWDTVVEITSIGDQHVFDGTV SGTHNFVANGISLHNSLEQDADVVILLHRPDAFDRDDPRGGEADFILAKHRNGPTKTVTVAHQLHLSRFANMAR

Human Homologue Blast Result

Alergen Protein

Link to Algpred
Non Allergen Predicted by AlgPred Server

Bacterial Toxin Prediction

Link to btxpred
No Hit Fountd by btxpred server.

Subcellular Location

Link to TBpred
Cyoplasmic

Antigens

No Hit Found in AntigenDB
No. Hit Found in IEDB

B cell Epitopes

BCEpred Analysis

Link to Bcepred
Result Predicited by [1]

ABCpred Analysis

Link to ABCpred
Result Predicited by [2]

IEDB Analysis

Link to IEDB
Result Predicited by [3]

MHC Class-I Binder

nHLAPred Analysis

Link to nHLApred
Result Predicited by [4]

IEDB Analysis

Link to IEDB
Result Predicted by []

MHC Class-II Binder

Propred Analysis

Link to Propred
Result Predicted by []

NetMHC-II Analysis

Link to NetMHC-II
Result Predicted by []

External Links

• Database of Mycobacterium tuberculosis genome sequences and related information.